Regularity and packing of the horizontal cell mosaic in different strains of mice.
Regularity and packing of the horizontal cell mosaic in different strains of mice.
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不同品系小鼠水平细胞嵌合体的规律性和堆积。
DOI：
10.1017/s0952523805224070
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Reese,BenjaminE
中科院分区：
文献类型：
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作者：
Raven,MaryA;Stagg,StephanieB;Reese,BenjaminE
本研究描述了四个品系的小鼠在水平细胞密度的两倍变化中，马赛克规律性、细胞间距和水平细胞堆积之间的关系。我们通过对数据集的进一步检查，测试了马赛克图案在密度变化中保持不变的预测，这是在我们最近证明细胞间距随着密度的增加而下降之后：对每个菌株的水平细胞的多个领域进行了最近邻和Voronoi域分析，并计算了它们各自的规则指数。对每个场进行自相关分析，生成密度恢复剖面，并计算有效半径和填充因子。整齐度指数与密度呈负相关，而不是保持恒定，表明细胞间距与密度之间的强负相关超过了产生简单的镶嵌鳞片所需的负相关。堆积因子与密度呈负相关，证实了这一点。这些结果表明，在这些菌株的水平细胞种群中出现的图案变化是作为密度函数控制细胞间距的表观遗传机制的结果。
The present study describes the relationships between mosaic regularity, intercellular spacing, and packing of horizontal cells across a two-fold variation in horizontal cell density in four strains of mice. We have tested the prediction that mosaic patterning is held constant across variation in density following our recent demonstration that intercellular spacing declines as density increases, by further examination of that dataset: Nearest-neighbor and Voronoi-domain analyses were conducted on multiple fields of horizontal cells from each strain, from which their respective regularity indices were calculated. Autocorrelation analysis was performed on each field, from which the density recovery profile was generated, and effective radius and packing factor were calculated. The regularity indexes showed negative correlations with density rather than being held constant, suggesting that the strong negative correlation between intercellular spacing and density exceeded that required to produce a simple scaling of the mosaic. This was confirmed by the negative correlation between packing factor and density. These results demonstrate that the variation in the patterning present in the population of horizontal cells across these strains is a consequence of epigenetic mechanisms controlling intercellular spacing as a function of density.