Growth of Chlamydia pneumoniae in cultured human peripheral blood mononuclear cells and induction of a cytokine response
Growth of Chlamydia pneumoniae in cultured human peripheral blood mononuclear cells and induction of a cytokine response
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DOI：
10.1006/mpat.1996.0056
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发表时间：
1996-09-01
影响因子：
3.8
通讯作者：
Leinonen, M
中科院分区：
文献类型：
--
作者：
KaukorantaTolvanen, SSE;Teppo, AM;Leinonen, M
在体外感染新鲜分离的人外周血单核细胞（HPBMC）与肺炎衣原体被发现诱导肿瘤坏死因子α（TNF-α），白细胞介素-1 β（IL-1 β），白细胞介素-6（IL-6）和干扰素α（IFN-α）的生产。分泌量与感染衣原体的数量有关，主要发生在感染后12 ~ 24 h。作为HPBMC活化的阳性对照，Mincobacterium Rechemotype的脂多糖（LPS）诱导TNF-α、IL-1 β和IL-6的释放，但不诱导IFN-α的释放，与C.肺炎。感染后立即分离的HPBMC不能回收活的衣原体，而感染前体外培养3 ~ 9天的HPBMC能够维持衣原体的生长。肺炎。HPBMCs内的生长以及诱导细胞因子应答可能在C.肺炎感染。(C)1996年学术出版社
In vitro infection of freshly isolated human peripheral blood mononuclear cells (HPBMC) with Chlamydia pneumoniae was found to induce a production of tumour necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and interferon alpha (IFN-alpha). The secretion was dependent on the amount of infecting chlamydiae and most of it occurred during the first 12 to 24 h. Lipopolysaccharide (LPS) of Salmonella minnesota Rechemotype, used as a positive control for HPBMC activation, induced a release of TNF-alpha, IL-1 beta and IL-6, but not of IFN-alpha, similar to the effect of C. pneumoniae. Viable chlamydiae could not be recovered from HPBMCs infected immediately after their isolation, whereas HPBMCs which were cultured in vitro for 3 to 9 days before infection were able to maintain the growth of C. pneumoniae. Growth inside HPBMCs as well as induction of cytokine response may have a role in the pathogenesis of C. pneumoniae infection. (C) 1996 Academic Press Limited