Regulation of glutamatergic signalling by PACAP in the mammalian suprachiasmatic nucleus.
Regulation of glutamatergic signalling by PACAP in the mammalian suprachiasmatic nucleus.
复制标题
PACAP在哺乳动物上核核中对谷氨酸能信号的调节。
DOI：
10.1186/1471-2202-7-15
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发表时间：
2006-02-16
期刊：
影响因子：
2.4
通讯作者：
Colwell, CS
中科院分区：
文献类型：
--
作者：
Michel, S;Itri, J;Han, JH;Gniotczynski, K;Colwell, CS
先前的研究表明，光信息通过含有谷氨酸和垂体腺苷酸环化酶激活肽（PACAP）的视网膜神经节细胞亚群到达视交叉上核（SCN）。虽然谷氨酸在这一途径中的作用已经得到了很好的研究，但PACAP及其受体的参与才刚刚开始被理解。推测PACAP可能调节视交叉上核神经元对谷氨酸的反应，我们使用电生理和钙成像工具来检查这些共递质的可能的细胞相互作用。外源性应用PACAP增加了小鼠脑切片制备中SCN神经元自发兴奋性突触后电流的振幅和频率。PACAP还通过PAC1受体和腺苷酸环化酶信号级联介导的机制增加了ampa诱发电流的强度。这种兴奋性电流的增强不仅限于AMPA引起的兴奋性电流，PACAP的应用也增强了NMDA电流的强度。此外，在大多数小鼠SCN神经元中，PACAP增强了AMPA和NMDA诱导钙瞬态，而PACAP单独对静息钙的影响很小。最后，在大鼠SCN神经元中，外源性PACAP增强了AMPA诱发电流和钙瞬态，并自身诱发了强健的钙瞬态。本文报道的结果表明，PACAP是夜间早期SCN内谷氨酸能信号的有效调节剂。
Previous studies indicate that light information reaches the suprachiasmatic nucleus (SCN) through a subpopulation of retinal ganglion cells that contain both glutamate and pituitary adenylyl cyclase activating peptide (PACAP). While the role of glutamate in this pathway has been well studied, the involvement of PACAP and its receptors are only beginning to be understood. Speculating that PACAP may function to modulate how neurons in the suprachiasmatic nucleus respond to glutamate, we used electrophysiological and calcium imaging tools to examine possible cellular interactions between these co-transmitters. Exogenous application of PACAP increased both the amplitude and frequency of spontaneous excitatory postsynaptic currents recorded from SCN neurons in a mouse brain slice preparation. PACAP also increased the magnitude of AMPA-evoked currents through a mechanism mediated by PAC1 receptors and the adenylyl cyclase-signalling cascade. This enhancement of excitatory currents was not limited to those evoked by AMPA as the magnitude of NMDA currents were also enhanced by application of PACAP. Furthermore, PACAP enhanced AMPA and NMDA evoked calcium transients while PACAP alone produced very little change in resting calcium in most mouse SCN neurons. Finally, in rat SCN neurons, exogenous PACAP enhanced AMPA evoked currents and calcium transients as well evoked robust calcium transients on its own. The results reported here show that PACAP is a potent modulator of glutamatergic signalling within the SCN in the early night.
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DOI：
10.1016/s0169-328x(02)00387-x
发表时间：
2002-09-30
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Ajpru, S;McArthur, AJ;Sugden, D
通讯作者：
Sugden, D
影响因子：
3.4
作者：
Bergström, AL;Hannibal, J;Fahrenkrug, J
通讯作者：
Fahrenkrug, J
DOI：
10.1073/pnas.96.23.13468
发表时间：
1999-11-09
影响因子：
11.1
作者：
Chen, D;Buchanan, GF;Gillette, MU
通讯作者：
Gillette, MU
影响因子：
2.9
作者：
Eggenberger, M;Born, W;Muff, R
通讯作者：
Muff, R
影响因子：
2.5
作者：
Itri, J;Colwell, CS
通讯作者：
Colwell, CS