HnRNP E2 is downregulated in human oral cancer cells and the overexpression of hnRNP E2 induces apoptosis
HnRNP E2 is downregulated in human oral cancer cells and the overexpression of hnRNP E2 induces apoptosis
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DOI：
10.1002/mc.20265
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发表时间：
2007-03-01
影响因子：
4.6
通讯作者：
Chaudhuri, Keya
中科院分区：
文献类型：
--
作者：
Roychoudhury, Paromita;Paul, Ranjan Rashmi;Chaudhuri, Keya
人类hnRNP基因已被报道参与人类恶性肿瘤，并且几种hnRNP是肺癌、头颈癌、结肠癌、乳腺癌和胰腺癌的有希望的生物标志物。本研究探讨hnRNPE 2基因在口腔癌中的表达及其临床病理学意义。实时荧光定量RT-PCR检测hnRNPE 2在口腔癌组织中的表达明显低于正常组织（P < 0.0001）。hnRNP E2的表达与组织学相关，与高分化鳞状细胞癌相比，在中度和低分化鳞状细胞癌（SCC）中的表达较低。在癌细胞系中瞬时转染hnRNP E2导致细胞活力降低和凋亡核增加。与对照转染子相比，具有更高表达的细胞通过膜联蛋白-PI染色显示凋亡细胞的数量增加，并且半胱天冬酶活性增加。因此，本研究暗示下调hnRNP E2作为一种新的机制，以提高癌细胞的耐凋亡。(c)2007 Wiley-Liss，Inc.
Human hnRNP genes have been reported to be involved in human malignancies and several hnRNPs are promising biomarkers of lung, head and neck, colon, breast, and pancreatic cancers. The present study investigated the clinicopathologic and biological significance of hnRNP E2 gene expression in oral cancer. Human hnRNP E2 was significantly downregulated in oral cancer tissues compared to normal one (P < 0.0001) as determined by quantitative real-time reverse transcription PCR. The expression of hnRNP E2 is correlated with histology, being lower in moderate and poorly differentiated squamous cell carcinoma (SCC) compared to well-differentiated SCC. Transient transfection of hnRNP E2 in cancerous cell lines resulted in reduced cell viability and increased apoptotic nuclei. Compared to control transfectants, cells with higher expression showed an increase in the number of apoptotic cells by annexin-PI staining and an increase in caspase activity. The present study thus implicates downregulation of hnRNP E2 as a novel mechanism to enhance the resistance of cancer cells to apoptosis. (c) 2007 Wiley-Liss, Inc.