Replicon-based vectors of positive strand RNA viruses.
Replicon-based vectors of positive strand RNA viruses.
复制标题
基于复制子的正链 RNA 病毒载体。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
A. Khromykh
中科院分区：
文献类型：
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作者：
A. Khromykh
基于正链RNA病毒自我复制RNA（复制子）的载体正在成为哺乳动物细胞中基因表达以及开发新型抗病毒和抗癌疫苗的强大工具。相对较小的基因组大小和简单的程序允许快速产生重组体。细胞质 RNA 扩增消除了细胞核的参与，导致极高水平的基因表达，双链 RNA 的连续合成可诱导增强的免疫反应，使这些载体在其他基因表达系统中独一无二。现在可以选择允许短期瞬时表达的细胞病变复制子载体和允许长期稳定表达的非细胞病变复制子载体，并根据特定应用选择载体。
Vectors based on self-replicating RNAs (replicons) of positive strand RNA viruses are becoming powerful tools for gene expression in mammalian cells and for the development of novel antiviral and anticancer vaccines. A relatively small genome size and simple procedure allow rapid generation of recombinants. Cytoplasmic RNA amplification eliminates nuclear involvement and leads to extremely high levels of gene expression, and continuous synthesis of double stranded RNA results in induction of enhanced immune responses, making these vectors unique among other gene expression systems. Both cytopathic replicon vectors allowing short-term transient expression, and non-cytopathic replicon vectors allowing long-term stable expression, are now available with the choice of vector depending on particular applications.