Nanopatterned cadmium selenide Langmuir-Blodgett platform for leukemia detection.
Nanopatterned cadmium selenide Langmuir-Blodgett platform for leukemia detection.
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DOI：
10.1021/ac202265a
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发表时间：
2012-03
影响因子：
7.4
通讯作者：
Aditya Sharma;C. M. Pandey;Zimple Matharu;U. Soni;S. Sapra;G. Sumana;M. Pandey;T. Chatterjee;B. Malhotra
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文献类型：
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作者：
Aditya Sharma;C. M. Pandey;Zimple Matharu;U. Soni;S. Sapra;G. Sumana;M. Pandey;T. Chatterjee;B. Malhotra
我们目前的研究结果有关的LB膜的制备三正辛基氧化膦封端的硒化镉量子点（QCdSe）到铟锡氧化物（ITO）涂层玻璃基板。研究了不同亚相条件下气-水界面的单分子层行为。这种纳米图案化的平台已被探索制造的电化学DNA生物传感器检测慢性粒细胞白血病（CML）的共价固定的巯基封端的寡核苷酸探针序列通过置换反应。电化学响应研究结果表明，该传感器可在120 s内检测10（-6）~ 10（-14）M范围内的目标DNA，保存期为2个月，可使用8次左右。此外，已发现该核酸传感器可区分CML阳性和对照阴性临床患者样品。
We present results of the studies relating to preparation of Langmuir-Blodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium-tin oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air-water interface under various subphase conditions. This nanopatterned platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by covalently immobilizing the thiol-terminated oligonucleotide probe sequence via a displacement reaction. The results of electrochemical response studies reveal that this biosensor can detect target DNA in the range of 10(-6) to 10(-14) M within 120 s, has a shelf life of 2 months, and can be used about 8 times. Further, this nucleic acid sensor has been found to distinguish the CML-positive and the control negative clinical patient samples.