The Tall Fescue Turf Grass Class I Chitinase Gene FaChit1 Is Activated by Fungal Elicitors, Dehydration, Ethylene, and Mechanical Wounding
The Tall Fescue Turf Grass Class I Chitinase Gene FaChit1 Is Activated by Fungal Elicitors, Dehydration, Ethylene, and Mechanical Wounding
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DOI：
10.1007/s11105-008-0086-8
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发表时间：
2009-02
影响因子：
2.1
通讯作者：
Jian Wang;N. Tian;Xuan Huang;Li Yu Chen;M. Schläppi;Ziqin Xu
中科院分区：
文献类型：
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作者：
Jian Wang;N. Tian;Xuan Huang;Li Yu Chen;M. Schläppi;Ziqin Xu
从高羊茅（Festuca arundinacea）中分离到一个Ⅰ类几丁质酶基因FaChit 1的cDNA、基因组DNA和启动子序列，并对其进行了结构分析。FaChit 1的氨基酸序列含有几丁质结合、催化、脯氨酸和甘氨酸丰富的结构域，但不含C端延伸区; FaChit 1可被真菌诱导子、脱水和乙烯有效诱导，而机械损伤仅轻微诱导。为了鉴定潜在的胁迫相关顺式作用元件，将FaChit 1起始密码子上游935、651和233 bp的5′序列与GUS报告基因融合，并在转基因烟草中进行分析。结果表明，935 bp的片段密切反映内源基因的表达和651 bp的片段是足够的直接报告基因的表达响应真菌诱导子，乙烯，脱水，或机械损伤，由于已知的和目前uncharacterizedcis-acting元件。
The cDNA, genomic DNA, and promoter sequence ofFaChit1, a class I chitinase gene fromFestuca arundinacea, were isolated and characterized in the present work. The deduced amino acid sequence of FaChit1 contains the chitin binding, catalytic, and proline and glycine-rich domains characteristic for most class I chitinases, but no C-terminal extension region.FaChit1is induced effectively by fungal elicitors, dehydration, and ethylene, but only slightly by mechanical wounding. To identify potential stress-relatedcis-acting elements, 5′ sequences 935, 651, and 233 bp upstream of theFaChit1start codon were fused to the GUS reporter gene and analyzed in transgenic tobacco. The results indicated that the 935 bp fragment closely mirrored endogenous gene expression and that the 651 bp fragment was sufficient to direct reporter the gene expression in response to fungal elicitors, ethylene, dehydration, or mechanical wounding due to both known and presently uncharacterizedcis-acting elements.