Role of p53 and p21 polymorphisms in the risk of cervical cancer among Chinese women
Role of p53 and p21 polymorphisms in the risk of cervical cancer among Chinese women
复制标题
p53 和 p21 多态性在中国女性宫颈癌风险中的作用。
DOI：
10.1093/abbs/gmq069
复制
发表时间：
2010-09-01
影响因子：
3.7
通讯作者：
Tang, Jianxin
中科院分区：
文献类型：
--
作者：
Jiang, Pei;Liu, Jianxin;Tang, Jianxin
本研究的目的是确定是否多态性变异的p53在密码子72和p21在密码子31与宫颈癌的风险增加，无论是独立或联合，在中国南方汉族妇女。我们对104名宫颈癌患者和160名对照者外周血DNA的p53密码子72和p21密码子31多态性进行了基因分型。基因分型通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和直接DNA测序进行确认。我们观察到与p53 Pro/Pro或p21 Arg/Arg基因型相比，p53 Arg/Arg（OR，2.25; 95% CI，1.11-4.54）或p21 Ser/Ser（OR，2.09; 95% CI，1.04-4.19）基因型与宫颈癌相关的风险增加。此外，p53和p21基因多态性之间的相互作用以倍增方式增加宫颈癌的风险，对于同时携带p53 Arg/Arg和p21 Ser/Ser基因型的受试者，OR为3.96（95%CI，1.51-10.41）。提示p53、p21基因多态性与中国南方妇女宫颈癌的发病风险存在显著相关性，在宫颈癌的发病中可能存在基因-基因交互作用。
The objective of this study was to identify whether polymorphic variants of p53 at codon 72 and p21 at codon 31 were associated with increased risk for cervical cancer, either independently or jointly, among Chinese women from southern Han. We genotyped p53 codon 72 and p21 codon 31 polymorphisms of peripheral blood DNA from 104 cervical cancer patients and 160 controls. Genotyping was confirmed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct DNA sequencing. We observed an increased risk of cervical cancer associated with the p53 Arg/Arg (OR, 2.25; 95% CI, 1.11-4.54) or p21 Ser/Ser (OR, 2.09; 95% CI, 1.04-4.19) genotype, compared with the p53 Pro/Pro or p21 Arg/Arg genotype, respectively. In additional, interaction between these p53 and p21 polymorphisms increased the risk of cervical cancer in a multiplicative manner, with the OR being 3.96 (95% CI, 1.51-10.41) for subjects carrying both p53 Arg/Arg and p21 Ser/Ser genotypes. These findings suggest that there is a significant association between the genetic polymorphism of p53, p21, and the risk of cervical cancer among Chinese southern women, and there is a possible gene-gene interaction in the incidence of cervical cancer.