Fluorescent polymeric transducer for the rapid, simple, and specific detection of nucleic acids at the zeptomole level
Fluorescent polymeric transducer for the rapid, simple, and specific detection of nucleic acids at the zeptomole level
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DOI：
10.1021/ja038900d
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发表时间：
2004-04-07
影响因子：
15
通讯作者：
Leclerc, M
中科院分区：
文献类型：
--
作者：
Doré, K;Dubus, S;Leclerc, M
我们报告了使用荧光聚噻吩生物传感器对几百个遗传物质分子的特异性检测。这种识别基于阳离子聚合物光学传感器与带负电的核酸靶标之间的简单静电相互作用，可以在不到1小时内完成，简单且经济实惠，并且不需要任何化学反应。这个简单的系统用途广泛，足以检测各种长度的核酸，包括流感病毒RNA基因组的一个片段。
We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done in less than 1 h, simply and affordably, and without any chemical reaction. This simple system is versatile enough to detect nucleic acids of various lengths, including a segment from the RNA genome of the Influenza virus.