Changes in structure and methylation pattern in a cluster of thymidine kinase genes.
Changes in structure and methylation pattern in a cluster of thymidine kinase genes.
复制标题
胸苷激酶基因簇的结构和甲基化模式的变化。
DOI：
10.1128/mcb.4.4.611-617.1984
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发表时间：
1984
影响因子：
5.3
通讯作者：
Scangos,GA
中科院分区：
文献类型：
--
作者：
Christy,BA;Scangos,GA
细胞系 101 是 Ltk- 的胸苷激酶 (TK) 阳性衍生物，其中含有约 101 个细胞。 20 个拷贝的单纯疱疹病毒 TK 基因排列成串联阵列。基因内三个位点和侧翼序列的 DNA 甲基化与表达呈负相关：101 系中的位点未甲基化，101 的 4 个 TK 阴性衍生物中的每个位点甲基化，以及源自它们的 21 个 TK 阳性衍生物中的每个位点未甲基化。 20 个 TK 基因拷贝中的大多数中，相同的三个位点受到影响，而它们之间的其他位点没有受到影响。尽管整个基因簇从未丢失，表明整合到基因组中是稳定的，但内部重排发生的频率很高。重排与甲基化状态或基因表达没有明显的相关性。
Cell line 101 is a thymidine kinase (TK)-positive derivative of Ltk−which contains ca. 20 copies of the herpes simplex virus TK gene organized in a tandem array. DNA methylation at three sites within the gene and flanking sequences was inversely correlated with expression: the sites were unmethylated in line 101, methylated in each of 4 TK-negative derivatives of 101, and unmethylated in each of 21 TK-positive derivatives derived from them. The same three sites were affected in most of the 20 copies of the TK gene, whereas other sites between them were not affected. Although the entire gene cluster was never lost, indicating that integration into the genome was stable, internal rearrangements occurred at a high frequency. The rearrangements had no obvious correlation with the state of methylation or with the expression of the genes.