In utero hematopoietic stem cell transplantation with haploidentical donor adult bone marrow in a canine model
In utero hematopoietic stem cell transplantation with haploidentical donor adult bone marrow in a canine model
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DOI：
10.1016/j.ajog.2004.01.014
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发表时间：
2004-04-01
影响因子：
9.8
通讯作者：
Jones, R
中科院分区：
文献类型：
--
作者：
Blakemore, K;Hattenburg, C;Jones, R
目的：在犬的子宫骨髓移植模型中，嵌合现象可以实现，每公斤大于或等于1 × 10(8)个CD34（+）单倍体相同的供体细胞，而不会出现移植物抗宿主病。研究设计：在超声引导下，以1.3 × 10(8)至2.5 × 10（10）个CD34（+）细胞/kg的剂量，从父亲骨髓中选择CD34+细胞，在30 ~ 41日龄的犬进行子宫内骨髓移植。建立了一种腹腔注射乙硫醇标记对照窝偶的方法。出生后的研究包括组织学、荧光原位杂交犬Y探针和基于聚合酶链反应的嵌合分析。结果：大于或等于34天的移植期生存率为86%至100%，而30天和31天的移植期生存率为14%和43%。Microchimerisin (
Objective: Chimerism, can be achieved in a canine model of in utero bone marrow transplantation with greater than or equal to 1 x 10(8) CD34(+) haploidentical donor cells per kilogram without graft-versus-host disease.Study design: In utero bone marrow transplantation was performed by ultrasound-guided intraperitoneal infusion in 30- to 41-day-old canines with CD34+ selected cells from paternal bone marrow at doses of 1.3 X 10(8) to 2.5 X 10(10) CD34(+) cells/kg. A method for marking control litter-mates was developed with intraperitoneal ethiodol. Postnatal studies included histologic, fluorescent in situ hybridization canine Y probe, and polymerase chain reaction-based chimerism analyses.Results: Term survival was 86% to 100% for transplantations greater than or equal to34 days versus 14% and 43 % at 30 and 31 days. Microchimerisin (