Activation of the neutrophil respiratory burst oxidase
Activation of the neutrophil respiratory burst oxidase
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DOI：
10.1086/513849
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发表时间：
1999-03-01
影响因子：
6.4
通讯作者：
Clark, RA
中科院分区：
文献类型：
--
作者：
Clark, RA
嗜中性粒细胞呼吸爆发氧化酶是一种多组分可活化酶，包括一种主要的吞噬细胞抗微生物系统。在遗传性疾病慢性肉芽肿病中，氧化酶功能缺失与复发性、严重且常常危及生命的感染有关。氧化酶系统的组分包括膜结合蛋白和可溶性胞质蛋白。刺激依赖性激活的一个主要特征是胞质因子复合物转移到膜上，在那里它们与黄细胞色素酶结合。各种氧化酶组分之间的相互作用通过许多特定区域发生，包括SH3结构域和富含脯氨酸的基序。完全组装的复合物作为电子传递系统发挥作用，将电子从细胞溶质NADPH移动到分子氧以形成超氧化物，超氧化物沿着随后的反应产物，发挥杀微生物和细胞毒性活性。
The neutrophil respiratory burst oxidase is a multicomponent activatable enzyme comprising one of the major phagocyte antimicrobial systems. In the genetic disorder chronic granulomatous disease, absent oxidase function is associated with recurrent, severe, and often life-threatening infections. The components of the oxidase system include both membrane-bound and soluble cytosolic proteins. A primary feature of stimulus-dependent activation is the translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme. Interactions among the various oxidase components occur through a number of specific regions, including SH3 domains and proline-rich motifs. The fully assembled complex functions as an electron transport system, moving electrons from cytosolic NADPH to molecular oxygen to form superoxide, which, along with subsequent reactive products, exerts microbicidal and cytotoxic activities.