Sperm IZUMO1-Dependent Gamete Fusion Influences Male Fertility in Mice
Sperm IZUMO1-Dependent Gamete Fusion Influences Male Fertility in Mice
复制标题
DOI：
10.3390/ijms20194809
复制
发表时间：
2019-09
影响因子：
5.6
通讯作者：
Takako Saito;I. Wada;Naokazu Inoue
中科院分区：
文献类型：
--
作者：
Takako Saito;I. Wada;Naokazu Inoue
精卵融合是通过每个配子中一组特定膜蛋白的相互作用完成的：精子IZUMO1和卵母细胞JUNO。最近，我们发现Izumo1基因的选择性剪接产生了一个新的Izumo1亚型（IZUMO1_v2）。在这里，我们获得了四个小鼠系，通过将原始IZUMO1 （IZUMO1_v1）敲除与IZUMO1-null （IZUMO1_v1和_v2均被破坏）遗传背景结合，获得了不同水平的IZUMO1蛋白，以确定IZUMO1的数量如何影响雄性生育能力。随后，我们通过western blot和小鼠IZUMO1单克隆抗体的免疫染色两种定量分析，明确了信号强度与平均产仔数密切相关。这些结果表明，评价IZUMO1蛋白水平有助于预测生殖力，是一种适用于雄性生殖力的检测方法。
Sperm–egg fusion is accomplished through the interaction of a specific set of membrane proteins in each gamete: sperm IZUMO1 and oocyte JUNO. Recently, we found that alternative splicing of the Izumo1 gene generates a novel IZUMO1 isoform (IZUMO1_v2). Here, we obtained four mouse lines, having graded different levels of IZUMO1 protein by combining an original IZUMO1 (IZUMO1_v1) knockout with IZUMO1-null (both IZUMO1_v1 and _v2 disrupted) genetic background, in order to determine how the quantity of IZUMO1 influences male fertility. Subsequently, we clarified that the signal intensity from two quantitative assays, western blot and immunostaining analyses with a monoclonal antibody against mouse IZUMO1, were strongly correlated with average litter size. These results suggest that evaluating IZUMO1 protein levels is useful for predicting fecundity, and is a suitable test for male fertility.