Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering
Spiral-structured, nanofibrous, 3D scaffolds for bone tissue engineering
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DOI：
10.1002/jbm.a.32591
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发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Yu, Xiaojun
中科院分区：
文献类型：
--
作者：
Wang, Junping;Valmikinathan, Chandra M.;Yu, Xiaojun
聚合物纳米纤维基质已广泛应用于组织工程领域。然而，由于目前的制造技术，将纳米纤维制造成复杂的三维(3D)结构受到限制。为了克服这一限制，我们将纳米纤维引入到由聚(己内酯)(PCL)制成的螺旋结构3D支架上。螺旋结构具有开放的几何结构、较大的表面积和孔隙率，将有助于改善营养物质的运输和细胞对支架的渗透，这在传统的组织工程支架修复大型骨缺损方面是有限的。为了研究结构和纤维涂层对支架性能的影响，制备了圆柱形PCL支架、螺旋PCL支架(无纤维涂层)和螺旋结构纤维PCL支架(有纤维涂层)。对支架的形貌、孔隙率和力学性能进行了表征。此外，将人成骨细胞种植在这些支架上，评估细胞在支架上的附着、增殖、分化和矿化基质沉积。结果表明，螺旋支架的孔隙率在人体松质骨范围内，具有适合细胞生长的孔结构，压缩弹性系数和强度明显低于圆柱形支架。与圆柱形支架相比，螺旋结构支架表现出促进细胞增殖、分化和矿化的能力，并允许更好的细胞生长和渗透。纳米纤维在螺旋支架上的结合进一步增强了细胞的附着、增殖和分化。这些研究表明，螺旋结构的纳米纤维支架有望成为骨组织工程应用的替代材料。(C)2009年Wiley期刊，Inc.《生物材料》93A：753-762,2010
Polymeric nanofiber matrices have already been widely used in tissue engineering. However, the fabrication of nanofibers into complex three-dimensional (3D) structures is restricted due to current manufacturing techniques. To overcome this limitation, we have incorporated nanofibers onto spiral-structured 3D scaffolds made of poly (epsilon-caprolactone) (PCL). The spiral structure with open geometries, large surface areas, and porosity will be helpful for improving nutrient transport and cell penetration into the scaffolds, which are otherwise limited in conventional tissue-engineered scaffolds for large bone defects repair. To investigate the effect of structure and fiber coating on the performance of the scaffolds, three groups of scaffolds including cylindrical PCL scaffolds, spiral PCL scaffolds (without fiber coating), and spiral-structured fibrous PCL scaffolds (with fiber coating) have been prepared. The morphology, porosity, and mechanical properties of the scaffolds have been characterized. Furthermore, human osteoblast cells are seeded on these scaffolds, and the cell attachment, proliferation, differentiation, and mineralized matrix deposition on the scaffolds are evaluated. The results indicated that the spiral scaffolds possess porosities within the range of human trabecular bone and an appropriate pore structure for cell growth, and significantly lower compressive modulus and strength than cylindrical scaffolds. When compared with the cylindrical scaffolds, the spiral-structured scaffolds demonstrated enhanced cell proliferation, differentiation, and mineralization and allowed better cellular growth and penetration. The incorporation of nanofibers onto spiral scaffolds further enhanced cell attachment, proliferation, and differentiation. These studies suggest that spiral-structured nanofibrous scaffolds may serve as promising alternatives for bone tissue engineering applications. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 753-762, 2010