DETECTION OF HERPESVIRUS-LIKE DNA-SEQUENCES IN KAPOSIS-SARCOMA IN PATIENTS WITH AND THOSE WITHOUT HIV-INFECTION
DETECTION OF HERPESVIRUS-LIKE DNA-SEQUENCES IN KAPOSIS-SARCOMA IN PATIENTS WITH AND THOSE WITHOUT HIV-INFECTION
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DOI：
10.1056/nejm199505043321801
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发表时间：
1995-05-04
影响因子：
158.5
通讯作者：
CHANG, Y
中科院分区：
文献类型：
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作者：
MOORE, PS;CHANG, Y
背景最近在卡波西肉瘤和获得性免疫缺陷综合征（AIDS）患者的病变中发现了疱疹病毒样DNA序列。目前尚不清楚这些序列是否也存在于经典的卡波西肉瘤或卡波西肉瘤中，卡波西肉瘤发生在人类免疫缺陷病毒（HIV）血清阴性的同性恋男性中。我们分析了艾滋病相关卡波西肉瘤患者、经典卡波西肉瘤患者和艾滋病毒血清阴性的卡波西肉瘤同性恋男性的组织样本中的DNA。我们还分析了这些患者的未受累组织样本和健康受试者的对照组织中的DNA。方法采用PCR方法，扩增疱疹病毒样DNA KS 330（233）序列。KS 330（233）PCR产物在卡波西肉瘤患者的21个组织样本中的20个（95%）中发现，其中包括艾滋病相关卡波西肉瘤患者的11个样本中的10个，经典卡波西肉瘤患者的所有6个样本，以及来自卡波西肉瘤的所有4个HIV阴性同性恋男性样本。21个对照样本中只有1个（5%）呈阳性（比值比，400; 95%置信区间，19至17，300）。在14例Kaposi肉瘤患者的未受累皮肤样本中，3例KS 330阳性（233）。三种类型卡波西肉瘤的代表性PCR产物序列超过98%相同，表明这三种类型的卡波西肉瘤是由同一种因子引起的。同样的疱疹病毒样DNA序列也存在于艾滋病相关的卡波西肉瘤、经典的卡波西肉瘤和发生在HIV阴性同性恋男性中的卡波西肉瘤中。因此，这种新的人类疱疹病毒可能不仅仅是艾滋病患者的机会性感染，卡波西肉瘤的三种形式可能是由同一种感染因子引起的。
Background. Herpesvirus-like DNA sequences have recently been found in lesions from patients with Kaposi's sarcoma and the acquired immunodeficiency syndrome (AIDS). It is not known whether these sequences are also present in classic Kaposi's sarcoma or in the Kaposi's sarcoma that occurs in homosexual men who are seronegative for the human immunodeficiency virus (HIV).Methods. We analyzed DNA in tissue samples from patients with AIDS-associated Kaposi's sarcoma, patients with classic Kaposi's sarcoma, and HIV-seronegative homosexual men with Kaposi's sarcoma. We also analyzed DNA in samples of uninvolved tissue from these patients and in control tissue from healthy subjects. All samples were tested blindly by polymerase chain reaction (PCR) with specific primers to amplify KS330(233), a herpesvirus-like DNA sequence.Results. The KS330(233) PCR product was found in 20 of 21 tissue samples (95 percent) from the patients with Kaposi's sarcoma, including 10 of the 11 samples from the patients with AIDS-associated Kaposi's sarcoma, all 6 samples from the patients with classic Kaposi's sarcoma, and all 4 samples from the HIV-negative homosexual men with Kaposi's sarcoma. Only 1 of the 21 control samples (5 percent) was positive (odds ratio, 400; 95 percent confidence interval, 19 to 17,300). Of the 14 samples of uninvolved skin from the patients with Kaposi's sarcoma, 3 were positive for KS330(233). Representative PCR-product sequences were more than 98 percent identical for the three types of Kaposi's sarcoma, suggesting that all three are caused by the same agent.Conclusions. The same herpesvirus-like DNA sequences are present in AIDS-associated Kaposi's sarcoma, classic Kaposi's sarcoma, and the Kaposi's sarcoma that occurs in HIV-negative homosexual men. Therefore, this presumably new human herpesvirus is not solely an opportunistic infection in patients with AIDS, and the three forms of Kaposi's sarcoma may be caused by the same infectious agent.