Functional analysis and intracellular localization of rice cryptochromes
Functional analysis and intracellular localization of rice cryptochromes
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DOI：
10.1104/pp.103.025759
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发表时间：
2003-12-01
期刊：
影响因子：
7.4
通讯作者：
Yamamoto, N
中科院分区：
文献类型：
--
作者：
Matsumoto, N;Hirano, T;Yamamoto, N
蓝光受体隐色素（CRY）介导子叶扩张、花青素积累增加和下胚轴伸长抑制，首次在拟南芥中被发现。两个拟南芥隐色素（AtCRY1和AtCRY2）被报道定位于细胞核。然而，目前还没有关于单子叶植物隐色素的资料。在这项研究中，我们从水稻（Oryza sativa）植株中分离了两个隐色素cdna， OsCRY1和OsCRY2。cscry1和CsCRY2的氨基酸序列在N端有一个类似光解酶的结构域，与AtCRY1同源。为了研究OsCRY1的功能，我们在拟南芥中过表达了绿色荧光蛋白-OsCRY1融合基因，并评估了由此产生的转基因植株的表型。当幼苗在黑暗中发芽时，没有观察到明显的影响。然而，光萌发的幼苗表现出明显的下胚轴伸长抑制和花青素积累增加。这些表型以蓝光依赖的方式诱导，表明OsCRY1作为蓝光受体隐色素起作用。我们还检测了绿色荧光蛋白- oscry1在转基因植物中的细胞内定位。它定位于细胞核和细胞质。我们在OsCRY1的初级结构中发现了两个核定位域。我们利用OsCRY2获得的额外数据讨论了水稻隐色素的功能与细胞内定位之间的关系。
Blue-light-receptor cryptochrome (CRY), which mediates cotyledon expansion, increased accumulation of anthocyanin, and inhibition of hypocotyl elongation, was first identified in Arabidopsis.. Two Arabidopsis cryptochromes (AtCRY1 and AtCRY2) have been reported to be localized to the nucleus. However, there is no information on the cryptochromes in monocotyledons. In this study, we isolated two cryptochrome cDNAs, OsCRY1 and OsCRY2, from rice (Oryza sativa) plants. The deduced amino acid sequences of OsCRY1 and CsCRY2 have a photolyase-like domain in their N termini and are homologous to AtCRY1. To investigate the function of OsCRY1, we overexpressed a green fluorescence protein-OsCRY1 fusion gene in Arabidopsis and assessed the phenotypes of the resulting transgenic plants. When the seedlings were germinated in the dark, no discernible effect was observed. However, light-germinated seedlings showed pronounced inhibition of hypocotyl elongation and increased accumulation of anthocyanin. These phenotypes were induced in a blue-light-dependent manner, indicating that OsCRY1 functions as a blue-light-receptor cryptochrome. We also examined the intracellular localization of green fluorescence protein-OsCRY1 in the transgenic plants. It was localized to both the nucleus and the cytoplasm. We identified two nuclear localization domains in the primary structure of OsCRY1. We discuss the relationship between the function and intracellular localization of rice cryptochromes by using additional data obtained with OsCRY2.