Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding
Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding
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DOI：
10.1002/elps.200305526
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发表时间：
2003-09
期刊：
影响因子：
2.9
通讯作者：
Jesper Østergaard;N. Heegaard
中科院分区：
文献类型：
--
作者：
Jesper Østergaard;N. Heegaard
毛细管电泳是研究非共价相互作用的成熟技术。存在多种方法，毛细管电泳-正面分析为迁移位移亲和毛细管电泳方法和传统方法提供了有趣的替代方法。本文回顾了建立正面分析方法的原理。本文讨论了毛细管电泳正面分析与传统和其他毛细管电泳定量结合相互作用方法的优势和局限性。利用毛细管电泳-正面分析的研究主要集中在药物与血浆蛋白的相互作用上。这些研究主要涉及药物与人血清白蛋白、α1 -酸性糖蛋白和脂蛋白的结合，以及毛细管电泳-正面分析方法的一些最新进展。
Capillary electrophoresis is a well‐established technique for the study of noncovalent interactions. Various approaches exist and capillary electrophoresis‐frontal analysis provides an interesting alternative to the migration shift affinity capillary electrophoresis methods and conventional methods. The present work reviews the principles on which the frontal analysis method is founded. Advantages and limitations of capillary electrophoresis frontal analysis in comparison with both conventional and other capillary electrophoresis based methods for quantification of binding interactions are discussed. Investigations utilizing capillary electrophoresis‐frontal analysis have focused on the interaction of drugs with plasma proteins. These studies, primarily addressing the binding of drugs to human serum albumin, α1‐acid glycoprotein, and lipoproteins are reviewed together with some recent developments in capillary electrophoresis‐frontal analysis methodology.