Capillary permeability of 131I-albumin in skeletal muscle.
Capillary permeability of 131I-albumin in skeletal muscle.
复制标题
骨骼肌中 131I-白蛋白的毛细血管通透性。
DOI：
10.1016/0026-2862(85)90018-4
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
O. Henriksen
中科院分区：
文献类型：
--
作者：
P. Sejrsen;W. Paaske;O. Henriksen
通过对自灌注猫腓肠肌制剂应用“单次注射、残留检测”方法测定骨骼肌中/sup 131/I-白蛋白的毛细血管通透性。零静脉压下的实验得出平均毛细管提取分数 E 为 0.028，血浆流量约为 3 ml X (100 g)-1 X min-1。渗透性-表面积乘积 PS 平均为 0.075 ml X (100 g)-1 X min-1。在 10 mm Hg 静脉压下进行的实验得到了基本相同的 PS 产品 (0.070 ml X (100 g)-1 X min-1。进一步的实验表明，不存在 /sup 131/I-白蛋白的血管内停滞池。纯化的 /sup 131/I-白蛋白在 37 度水中的自由扩散系数为 0.0631 X 10-5 cm2 X秒-1）。 /sup 131/I-白蛋白在间隙空间中的分布体积为3.5ml X (100g)-1 X sec-1。假设毛细血管表面积为 70 cm2 X g-1，渗透系数计算为 18 X 10(-8) cm X sec-1，比淋巴取样方法获得的值高约 3 至 10 倍。结果表明，/sup 131/I-白蛋白的主要跨毛细管转运机制是扩散。根据受限扩散理论进行分析时，结果与通过有效等效孔隙半径为 145 A 的孔隙的跨毛细管扩散相一致。
Capillary permeability of /sup 131/I-albumin in skeletal muscle was determined by applying the ''single injection, residue detection'' method to the autoperfused cat gastrocnemius muscle preparation. Experiments at zero venous pressure gave an average capillary extraction fraction, E, of 0.028 at a plasma flow of about 3 ml X (100 g)-1 X min-1. The permeability-surface area product, PS, was on average 0.075 ml X (100 g)-1 X min-1. Experiments at a venous pressure of 10 mm Hg gave essentially the same PS product (0.070 ml X (100 g)-1 X min-1. Further experiments demonstrated that stagnant intravascular pools of /sup 131/I-albumin were not present. The free diffusion coefficient in water at 37 degrees of the purified /sup 131/I-albumin was 0.0631 X 10-5 cm2 X sec-1). The distribution volume for /sup 131/I-albumin in the interstitial space was 3.5 ml X (100 g)-1 X sec-1. Assuming a capillary surface area of 70 cm2 X g-1 the permeability coefficient was calculated to be 18 X 10(-8) cm X sec-1 which is about 3 to 10 times higher than values obtained by lymph sampling methods. The results indicate that the predominant transcapillary transport mechanism for /sup 131/I-albumin is diffusion. When analyzed according to the theory of restricted diffusion the results are compatible with transcapillary diffusion through pores with an effective equivalent pore radius of 145 A.