Furosemide accelerates gentamicin accumulation in cultured renal cells (LLC-PK1 cells).
Furosemide accelerates gentamicin accumulation in cultured renal cells (LLC-PK1 cells).
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DOI：
10.1159/000185726
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发表时间：
1989
期刊：
影响因子：
2.5
通讯作者：
H. Nakahama;Y. Fukuhara;Y. Orita;A. Yamauchi;T. Takama;T. Kamada
中科院分区：
文献类型：
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作者：
H. Nakahama;Y. Fukuhara;Y. Orita;A. Yamauchi;T. Takama;T. Kamada
已知速尿可增强庆大霉素的肾毒性。增强的机制尚不清楚。在我们之前的研究中，我们证明了呋塞米作为一种丸剂注射在兔肾组织中可以增强庆大霉素的积累。33:363, 1988]或反复皮下注射[利尿剂II， Elsevier, New York, 1987, pp.693]。在这项研究中，我们评估了速尿对培养肾细胞（LLC-PK1）和肝癌细胞（H4IIE）庆大霉素积累的影响。因此，我们排除了呋塞米引起的血流动力学改变的继发效应。播种后第7天，将培养基换成含有1mm庆大霉素[G]或1mm庆大霉素+ 1mm呋塞米[GF]的培养基。在lc - pk1细胞中，[GF]中庆大霉素浓度显著高于[G]，而在加或不加速尿的H4IIE细胞中均未检测到庆大霉素。我们得出结论，速尿可加速庆大霉素在肾组织中的蓄积，增强庆大霉素的肾毒性。
Furosemide is known to potentiate gentamicin nephrotoxicity. The mechanism of potentiation is unclear. In our previous studies, we demonstrated that furosemide enhanced gentamicin accumulation in rabbit renal tissues when injected as a bolus [Kidney int. 33:363, 1988] or repeatedly subcutaneously [Diuretics II, Elsevier, New York 1987, pp.693]. In this study, we evaluated the effects of furosemide on gentamicin accumulation in cultured renal cells (LLC-PK1) and hepatoma cells (H4IIE). Thus, we excluded effects secondary to furosemide-elicited hemodynamic changes. Seven days after seeding, the culture medium was exchanged for medium containing 1 mM gentamicin alone [G] or 1 mM gentamicin +1 mM furosemide [GF]. In LLC-PK1 cells, gentamicin concentration in [GF] was significantly higher than that in [G], while gentamicin was not detected in H4IIE cells with or without furosemide. We conclude that furosemide can accelerate gentamicin accumulation in renal tissues and potentiate gentamicin nephrotoxicity.