MiR-374b-5p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity
MiR-374b-5p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity
复制标题
DOI：
10.1155/2020/8024763
复制
发表时间：
2020-08-24
影响因子：
--
通讯作者：
Zhao, Wei
中科院分区：
文献类型：
--
作者：
Li, Dongjie;Du, Xiancai;Zhao, Wei
囊状棘球蚴病是由细粒棘球蚴（Echinococcus granulosus，Eg）感染引起的人畜共患疾病。本实验室前期研究证实了重组Eg. P29（rEg. P29）对绵羊和小鼠继发感染细粒棘球绦虫具有保护作用。本研究旨在探讨mmu-miR-374 b-5 p（miR-374 b-5 p）与rEg.P29疫苗免疫保护作用的相关性，以及miR-374 b-5 p对小鼠脾脏CD 4（+）T细胞免疫功能的影响。在用rEg.P29疫苗接种的第二周和第四周后，MiR-374 b-5 p水平显著增加。miR-374 b-5 p的过表达增加了CD 4（+）T细胞中IFN-γ、IL-2、IL-17 A mRNA水平，并降低了IL-10 mRNA水平。此外，miR-374 b-5 p的抑制降低了IFN-γ和IL-17 A，并增加了CD 4（+）T细胞中IL-10的mRNA水平;这通过流式细胞术进一步证实。接种rEg.P29增强了miR-374 b-5 p表达，这与较高的Th 1和Th 17免疫应答、较低的IL-10 mRNA产生（伴随miR-374 b-5 p过表达）、较低的Th 1免疫应答以及较高的IL-10 mRNA水平（伴随miR-374 b-5 p抑制）相关。综上所述，这些数据表明miR-374 b-5 p可能通过调节Th 1和Th 17分化参与rEg.P29免疫。
Cystic echinococcosis(CE) is a zoonotic disease caused byEchinococcus granulosus(Eg) infection. Our previous study confirmed that recombinant Eg.P29 (rEg.P29) could protect againstechinococcus granulosussecondary infection in sheep and mice. The aim of the study was to investigate the association between immunoprotection of rEg.P29 vaccine and mmu-miR-374b-5p (miR-374b-5p) and study the immunity influence of miR-374b-5p on CD4(+)T cells in mice spleen. MiR-374b-5p level was significantly increased after the second-week and the fourth week of vaccination with rEg.P29. Overexpression of miR-374b-5p increased IFN-gamma, IL-2, IL-17A mRNA levels and decreased IL-10 mRNA levels in CD4(+)T cells. Moreover, the inhibition of miR-374b-5p decreased IFN-gamma and IL-17A and increased IL-10 mRNA levels in CD4(+)T cells; this was further confirmed by the flow cytometry. The vaccination of rEg.P29 enhanced miR-374b-5p expression that was associated with a higher Th1 and Th17 immune response, a lower IL-10 mRNA production with miR-374b-5p overexpression, a lower Th1 immune response, and a higher IL-10 mRNA levels with miR-374b-5p inhibitions. To sum up, these data suggest that miR-374b-5p may participate in rEg.P29 immunity by regulating Th1 and Th17 differentiation.