Identification of an RNA silencing suppressor encoded by a mastrevirus
Identification of an RNA silencing suppressor encoded by a mastrevirus
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乳腺病毒编码的 RNA 沉默抑制子的鉴定
DOI：
10.1099/vir.0.064246-0
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发表时间：
2014
影响因子：
3.8
通讯作者：
Zhou Xueping
中科院分区：
文献类型：
--
作者：
Wang Yaqin;Dang Mingqing;Hou Huwei;Mei Yuzhen;Qian Yajuan;Zhou Xueping
小麦矮缩病毒(WDV)是双生病毒科MastreVirus属的一种DNA病毒。在本研究中，我们报道了WDV编码的Rep蛋白是一种RNA沉默抑制因子，通过使用携带GFP报告基因的转基因烟碱16c进行共渗透试验确定。Rep蛋白被证明既能抑制GFP基因的局部和系统RNA沉默，也能抑制系统GFP RNA沉默信号的传播。凝胶迁移率分析表明，Rep蛋白结合了21和24个核苷酸的小干扰RNA(SiRNA)双链和单链(Ss)-siRNA。据我们所知，这是首次发现由巨型病毒编码的RNA沉默抑制因子。此外，缺失突变表明，Rep蛋白的N端和C端区域对沉默抑制和自我相互作用不是关键的，但N端是其致病所必需的。
Wheat dwarf virus (WDV) is a DNA virus belonging to the genusMastrevirusof the familyGeminiviridae. In this study, we report that the Rep protein encoded by WDV is a RNA silencing supressor as determined by co-infiltration assays using transgenicNicotiana benthamianaline 16c carrying the GFP reporter gene. The Rep protein was shown to inhibit both local and systemic RNA silencing of the GFP gene as well as the spread of systemic GFP RNA silencing signals. Gel mobility shift assays showed that the Rep protein binds 21 nt and 24 nt small interfering RNA (siRNA) duplexes and single-stranded (ss)-siRNA. To our knowledge, this is the first identification of an RNA silencing suppressor encoded by mastreviruses. Furthermore, deletion mutagenesis indicates that both the N- and C-terminal regions of the Rep protein are not critical for silencing suppression and self-interaction, but the N terminus of Rep is necessary for its pathogenicity.