Homology modelling of the major peanut allergen Ara h 2 and surface mapping of IgE-binding epitopes
Homology modelling of the major peanut allergen Ara h 2 and surface mapping of IgE-binding epitopes
复制标题
DOI：
10.1016/j.imlet.2005.03.014
复制
发表时间：
2005-09-15
影响因子：
4.4
通讯作者：
Rougé, P
中科院分区：
文献类型：
--
作者：
Barre, A;Borges, JP;Rougé, P
花生阿糖胞苷h 2过敏原和其他结构相关的2S白蛋白过敏原的膳食坚果的三维模型，表现出整体的三维折叠稳定的二硫桥之间的所有成员的2S白蛋白超家族保守。构象分析的线性IgE结合表位映射的分子表面上的Ara h 2显示没有结构同源性与相应的区域的核桃Jug r 1，山核桃坚果车i 1或巴西坚果Ber e 1过敏原。没有表位社区不支持花生和核桃或巴西坚果之间观察到的过敏性交叉反应，这可能取决于其他无处不在的种子贮藏蛋白过敏原，即豌豆毒蛋白。然而，核桃Jug r 1过敏原的分子表面上鉴定的主要IgE结合表位与山核桃坚果Car i I过敏原的相应区域具有明显的结构同源性。因此，除了花生之外，2S白蛋白可以解释在一些其他膳食坚果（例如核桃和山核桃）之间观察到的IgE结合交叉反应性。(c)2005 Elsevier B.V.保留所有权利。
Three-dimensional models built for the peanut Ara h 2 allergen and other structurally-related 2S albumin allergens of dietary nuts exhibited an overall three-dimensional fold stabilized by disulphide bridges well conserved among all the members of the 2S albumin superfamily. Conformational analysis of the linear IgE-binding epitopes mapped on the molecular surface of Ara h 2 showed no structural homology with the corresponding regions of the walnut Jug r 1, the pecan nut Car i 1 or the Brazil nut Ber e 1 allergens. The absence of epitopic community does not support the allergenic cross-reactivity observed between peanut and walnut or Brazil nut, which presumably depends on other ubiquitous seed storage protein allergens, namely the vicilins. However, the major IgE-binding epitope identified on the molecular surface of the walnut Jug r 1 allergen shared a pronounced structural homology with the corresponding region of the pecan nut Car i I allergen. With the exception of peanut, 2S albumins could thus account for the IgE-binding cross-reactivity observed between some other dietary nuts, e.g. walnut and pecan nut. (c) 2005 Elsevier B.V. All rights reserved.