Archaeal diversity in naturally occurring and impacted environments from a tropical region
Archaeal diversity in naturally occurring and impacted environments from a tropical region
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DOI：
10.1111/j.1365-2672.2006.03230.x
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发表时间：
2007-07-01
影响因子：
4
通讯作者：
Martins, O. B.
中科院分区：
文献类型：
--
作者：
Clementino, M. M.;Fernandes, C. C.;Martins, O. B.
目的：评估南美洲热带地区巴西里约热内卢州自然和受影响栖息地的古菌多样性。方法和结果：通过聚合酶链式反应 (PCR) 从基因组 DNA 中直接扩增 16S rRNA 基因，基因组 DNA 提取自瓜纳巴拉湾 (GB) 水、卤素沉积物 (HS)、城市垃圾填埋场渗滤液、农业土壤和废水处理 (WT) 系统。构建了 5 个古菌 16S rDNA 克隆文库。在所分析的 5 个文库中，总共 123 个克隆被聚类为 29 个可操作的分类单元，与栽培 (24%) 和未栽培 (76%) 生物体相关。稀疏分析表明文库包含不同程度的多样性。 16S-23S 基因间隔区的 PCR 变性梯度凝胶电泳 (DGGE) 证实了 WT 系统克隆库揭示的显性系统发育型的存在。结论：受影响环境的古菌群落似乎仅限于具有相似理化特性的特定生态系统，而来自自然环境的群落似乎分布广泛。大量与未开化生物相关的系统发育型表明了新的古菌谱系。 这项研究的意义和影响：这项研究首次报告了对巴西热带环境中古菌多样性的分析，并将该地区的序列添加到正在开发的环境样本 16S rRNA 克隆文库数据库中。
Aims: To evaluate archaeal diversity in natural and impacted habitats from Rio de Janeiro state, Brazil, a tropical region of South America.Methods and Results: 16S rRNA gene was amplified directly by polymerase chain reaction (PCR) from genomic DNA, extracted from Guanabara Bay (GB) water, halomarine sediment (HS), municipal landfill leachate, agricultural soil and wastewater treatment (WT) system. Five archaeal 16S rDNA clone libraries were constructed. A total of 123 clones, within the five libraries analysed, were clustered into 29 operational taxonomic units, related to cultivated (24%) and uncultivated (76%) organisms. Rarefaction analysis showed that the libraries contained different levels of diversity. PCR-denaturing gradient gel electrophoresis (DGGE) of 16S-23S intergenic spacer regions confirmed the presence of a dominant phylotype, revealed by the WT system clone library.Conclusions: Archaeal communities of impacted environments seem to be confined to specific ecosystems with similar physicochemical properties, while communities from natural environments appear to be widely distributed. The presence of a high number of phylotypes related to uncultivated organisms suggests new archaeal lineages.Significance and Impact of the Study: This study reports, for the first time, the analysis of archaeal diversity in tropical environments from Brazil, and adds sequences from this region to the developing database of 16S rRNA clone libraries from environmental samples.