Decreased absolute counts of T lymphocyte subsets and their relation to disease in squamous cell carcinoma of the head and neck
Decreased absolute counts of T lymphocyte subsets and their relation to disease in squamous cell carcinoma of the head and neck
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DOI：
10.1158/1078-0432.ccr-04-0054
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发表时间：
2004-06-01
影响因子：
11.5
通讯作者：
Whiteside, TL
中科院分区：
文献类型：
--
作者：
Kuss, I;Hathaway, B;Whiteside, TL
目的：头颈部鳞状细胞癌患者外周血中CD8+T细胞的凋亡[SCCHN(Hoffmann T，et al.临床癌研究报告2002；8：255362)]提示淋巴细胞失衡的可能性。因此，检测SCCHN患者和对照组外周血中淋巴细胞亚群的绝对数和百分比。实验设计：采集146例SCCHN患者和54名正常志愿者的静脉血。采用基于流式细胞术的荧光标记技术测定CD3+、CD4+和CD8+T淋巴细胞的绝对数。用流式细胞仪检测T淋巴细胞亚群的百分率。结果：SCCHN患者外周血中CD3+、CD4+、CD8+T细胞绝对数明显低于正常对照组。然而，T细胞亚群的百分比在患者和正常对照组之间没有观察到差异。活动期疾病患者的CD3+和CD4+T细胞计数明显低于NED患者。手术和放疗后发生NED的患者在NED队列中T细胞计数最低。患有NED 2年的患者T细胞计数没有恢复，T细胞失衡在根治手术后多年明显。肿瘤转移(TNM)分期或病变部位与T细胞绝对计数无关。结论：SCCHN患者存在淋巴细胞动态平衡的改变，这种改变可持续数月或数年。
Purpose: Apoptosis of circulating CD8+ T cells seen in patients with squamous; cell carcinoma of the head and neck [SCCHN (Hoffmann T, et al. Clin Cancer Res 2002;8:255362)] suggested a possibility of lymphocyte imbalance. Therefore, absolute numbers and percentages of lymphocyte subsets were examined in the peripheral blood of SCCHN patients and controls.Experimental Design: Venous blood was obtained from 146 patients with SCCHN and 54 normal volunteers. Absolute numbers of CD3+, CD4+, and CD8+ T lymphocytes were determined using fluorobeads in a flow cytometry-based technique. Percentages of T lymphocyte subsets were also evaluated by flow cytometry. The patients were grouped at the time of blood draw [active versus no evidence of disease (NED), type of therapy administered, and the length of follow-up].Results: Patients with SCCHN had significantly lower absolute numbers of CD3+ CD4+, and CD8+ T cells than normal controls. However, no differences in the percentages of T-cell subsets between patients and normal controls were observed. Patients with active disease had significantly lower CD3+ and CD4+ T-cell counts than those with NED. Patients who had NED after surgery and radiotherapy had the lowest T-cell counts among the NED cohort. Patients who had NED for >2 years did not recover their T-cell counts, and the T-cell imbalance was evident many years after curative surgery. The tumor-node-metastasis (TNM) stage or site of the disease was not related to the absolute T-cell count. Patients with recurrent disease at the time of blood draw tended to have the lowest CD4+ T-cell counts.Conclusions: Patients with SCCHN have altered lymphocyte homeostasis, which persists for months or years after curative therapies.