Structural elucidation of the O-antigen of the Shigella flexneri provisional serotype 88-893: structural and serological similarities with S. flexneri provisional serotype Y394 (1c)
Structural elucidation of the O-antigen of the Shigella flexneri provisional serotype 88-893: structural and serological similarities with S. flexneri provisional serotype Y394 (1c)
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DOI：
10.1016/j.carres.2011.02.013
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发表时间：
2011-05-01
影响因子：
3.1
通讯作者：
Widmalm, G.
中科院分区：
文献类型：
--
作者：
Foster, R. A.;Carlin, N. I. A.;Widmalm, G.
福氏志贺菌暂定血清型88-893的O-抗原多糖的重复单元的结构已被确定。(1)H用~（13）C核磁共振谱和二维核磁共振谱对化合物的结构进行了表征。六糖重复单元的碳水化合物部分与先前阐明的来自S的O-多糖的结构相同。福氏杆菌血清型Y394。S.如通过H2 BC和BS-CT-HMBC NMR实验所鉴定的，福氏血清型88-893在位于3-取代的鼠李糖残基的O-2处的每个重复单元携带0.7 mol O-乙酰基。0-抗原多糖由具有以下结构的六糖重复单元组成：-> 2）-α-L-Rhap-（1 -> 2）-α-L-Rhap-（1 -> 3）-α-L-Rhap 2Ac-（1 -> 3）[α-D-Glcp-（1 -> 2）-α-D-Glcp（1 -> 4）]-β-D-GlcpNAc-（1 ->.血清学研究表明，两种暂定血清型的型抗原是相同的;此外，88-893表达S。弗氏群因子6抗原。我们建议将暂定血清型Y394和88-893分别命名为两个新的血清型7a和7 b。flexneri打字方案。(C)2011爱思唯尔有限公司保留所有权利。
The structure of the repeating unit of the O-antigen polysaccharide from Shigella flexneri provisional serotype 88-893 has been determined. (1)H and (13)C NMR spectroscopy as well as 2D NMR experiments were employed to elucidate the structure. The carbohydrate part of the hexasaccharide repeating unit is identical to the previously elucidated structure of the O-polysaccharide from S. flexneri prov. serotype Y394. The O-antigen of S. flexneri prov. serotype 88-893 carries 0.7 mol O-acetyl group per repeating unit located at O-2 of the 3-substituted rhamnosyl residue, as identified by H2BC and BS-CT-HMBC NMR experiments. The O-antigen polysaccharide is composed of hexasaccharide repeating units with the following structure: -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 3)-alpha-L-Rhap2Ac-(1 -> 3)[alpha-D-Glcp-(1 -> 2)-alpha-D-Glcp(1 -> 4)]-beta-D-GlcpNAc-(1 ->. Serological studies showed that type antigens for the two provisional serotypes are identical; in addition 88-893 expresses S. flexneri group factor 6 antigen. We propose that provisional serotypes Y394 and 88-893 be designated as two new serotypes 7a and 7b, respectively, in the S. flexneri typing scheme. (C) 2011 Elsevier Ltd. All rights reserved.