In vitro assays for activities of retroviral integrase
In vitro assays for activities of retroviral integrase
复制标题
DOI：
10.1006/meth.1997.0484
复制
发表时间：
1997-08-01
期刊：
影响因子：
--
通讯作者：
Chow, SA
中科院分区：
文献类型：
--
作者：
Chow, SA
逆转录病毒DNA的整合是逆转录病毒生命周期中的一个重要步骤，由病毒蛋白整合酶介导。描述了用于测量整合酶活性的简单体外测定法，包括催化（3 '端加工、3'端连接、解体）、病毒DNA末端的并置、DNA结合和靶位点选择。所描述的测定将有助于阐明逆转录病毒整合的分子机制和筛选整合酶抑制剂作为潜在的抗逆转录病毒药物。(C)北京：科学出版社.
Integration of retroviral DNA, an essential step during the retroviral life cycle, is mediated by the viral protein integrase. Simple in vitro assays for measuring integrase activities are described, including catalysis (3'-end processing, 3'-end joining, disintegration), juxtaposition of viral DNA ends, DNA binding, and target site selection. The described assays will be useful in elucidating the molecular mechanism of retroviral integration and screening for integrase inhibitors as potential anti-retroviral drugs. (C) 1997 Academic Press.