Drosophila regulatory factor X is necessary for ciliated sensory neuron differentiation
Drosophila regulatory factor X is necessary for ciliated sensory neuron differentiation
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DOI：
10.1242/dev.00148
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发表时间：
2002-12-01
期刊：
影响因子：
4.6
通讯作者：
Durand, B
中科院分区：
文献类型：
--
作者：
Dubruille, R;Laurençon, A;Durand, B
纤毛神经元在许多动物的感觉知觉中起着重要作用。树突末端的修饰纤毛是感觉信号捕获和转导的场所。我们描述了影响转录因子RFX的果蝇突变和遗传拯救实验，证明其在感觉纤毛分化中的核心作用。Rfx突变果蝇在化学感觉和机械感觉行为上存在缺陷，但趋光性正常，这与Rfx在纤毛感觉神经元和神经元前体中的表达一致，而在光感受器中不表达。突变的行为表型与神经元纤毛的功能和结构异常有关，表现为感觉转导的丧失以及纤毛形态和超微结构的缺陷。这些结果表明Rfx是果蝇纤毛感觉神经元分化的重要调节因子。
Ciliated neurons play an important role in sensory perception in many animals. Modified cilia at dendrite endings serve as sites of sensory signal capture and transduction. We describe Drosophila mutations that affect the transcription factor RFX and genetic rescue experiments that demonstrate its central role in sensory cilium differentiation. Rfx mutant flies show defects in chemosensory and mechanosensory behaviors but have normal phototaxis, consistent with Rfx expression in ciliated sensory neurons and neuronal precursors but not in photoreceptors. The mutant behavioral phenotypes are correlated with abnormal function and structure of neuronal cilia, as shown by the loss of sensory transduction and by defects in ciliary morphology and ultrastructure. These results identify Rfx as an essential regulator of ciliated sensory neuron differentiation in Drosophild.