The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein
The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein
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DOI：
10.1039/c4cc09287b
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Konkolewicz, Dominik
中科院分区：
文献类型：
--
作者：
Falatach, Rebecca;McGlone, Cameron;Konkolewicz, Dominik
通过使用 RAFT 聚合的接枝到和接枝方法的组合，将亲水性聚合物附着到溶菌酶上。合成亲水性低聚物，并将其附着到蛋白质上。蛋白质-寡聚物杂化物含有 RAFT 端基，可在溶液中进行链延伸。溶菌酶在整个过程中保持活性。
Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein-oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.