Antisense Oligonucleotides with Different Backbones
Antisense Oligonucleotides with Different Backbones
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具有不同骨架的反义寡核苷酸
DOI：
--
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发表时间：
1999
影响因子：
4.8
通讯作者：
R. Kole
中科院分区：
文献类型：
--
作者：
G. Schmajuk;H. Sierakowska;R. Kole
一种新的，阳性读出分析，定量仅序列特异性核活性的反义寡核苷酸被用于评估morpholino和2 ' -o -甲基糖-磷酸寡核苷酸。该检测是基于人β-珠蛋白pre-mRNA剪接途径的修饰。此外，用寡核苷酸刮载细胞可以单独评估细胞内寡核苷酸的反义活性及其穿透细胞膜屏障的能力。结果表明，在刮刮负载下，morpholino寡核苷酸的固有反义活性比磷酸二酯/硫代磷2 ' -o -甲基寡核苷酸的反义活性高约3倍，比纯硫代磷和磷酸二酯衍生物的反义活性分别高6-9倍和近200倍。morolino寡核苷酸比2 ' -o -甲基寡核苷酸从培养基中游离吸收的效率高20倍以上。在单层HeLa细胞和悬浮K562细胞中均能检测到morpholino寡核苷酸的反义活性。时间过程实验表明，morpholino寡核苷酸的自由摄取和外排都是缓慢的。
A novel, positive read-out assay that quantifies only sequence-specific nuclear activity of antisense oligonucleotides was used to evaluate morpholino and 2′-O-methyl sugar-phosphate oligonucleotides. The assay is based on modification of the splicing pathway of human β-globin pre-mRNA. In addition, scrape-loading of cells with oligonucleotides allows the separate assessment of intracellular antisense activity of the oligonucleotides and their ability to penetrate the cell membrane barrier. The results show that, with scrape-loading, the morpholino oligonucleotides were approximately 3-fold more effective in their intrinsic antisense activity than alternating phosphodiester/phosphorothioate 2′-O-methyl-oligoribonucleotides and 6–9- and almost 200-fold more effective than the exclusively phosphorothioate and phosphodiester derivatives, respectively. The morpholino oligonucleotides were over 20-fold more effective than the phosphorothioate 2′-O-methyl-oligoribonucleotides in free uptake from the culture media. The antisense activity of the morpholino oligonucleotides was detectable not only in monolayer HeLa cells but also in suspension K562 cells. Time course experiments suggest that both the free uptake and efflux of morpholino oligonucleotides are slow.
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影响因子：
2.9
作者：
Kang, SH;Cho, MJ;Kole, R
通讯作者：
Kole, R
硫代磷酸寡脱氧核苷酸对体外血管平滑肌细胞增殖、迁移和体内新内膜形成的序列独立抑制。
DOI：
10.1172/jci118810
发表时间：
1996
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Wang,W;Chen,HJ;Schwartz,A;Cannon,PJ;Stein,CA;Rabbani,LE
通讯作者：
Rabbani,LE
体内剪接位点对反义寡核苷酸的敏感性。
DOI：
10.1017/s135583829998130x
发表时间：
1999
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Sierakowska,H;Sambade,MJ;Schümperli,D;Kole,R
通讯作者：
Kole,R
突变型人 β-珠蛋白基因中 IVS 2 错误剪接的可逆性。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Dobkin,C;Bank,A
通讯作者：
Bank,A
影响因子：
20.3
作者：
Lewis, J;Yang, BL;Maeda, N
通讯作者：
Maeda, N