A REVERSIBLE PHOTOREACTION CONTROLLING SEED GERMINATION
A REVERSIBLE PHOTOREACTION CONTROLLING SEED GERMINATION
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DOI：
10.1073/pnas.38.8.662
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发表时间：
1952-01-01
影响因子：
11.1
通讯作者：
TOOLE, VK
中科院分区：
文献类型：
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作者：
BORTHWICK, HA;HENDRICKS, SB;TOOLE, VK
利用色散约为35 a /cm的双棱镜（玻璃）光谱，获得了促进和抑制大急流城莴苣种子萌发的作用谱。6000华氏度。在该波长处狭缝宽度约为90a，入射能量为0.100 x 10-3焦耳/cm2./sec。生菜种子放在盘子里的湿吸墨纸上，在20[度]C的黑暗中保持不同时间，然后照射。在5800到6600 A的辐射范围内（对照组的发芽率为98+%），对这些被抑制的种子进行辐射，然后在不同的波长区域进行辐射，以测试其抑制作用。那些促进发芽的种子直接从黑暗中取出并照射。辐照后，所有种子在20℃的黑暗条件下保存2天，然后进行发芽计数。种子在4 ~ 16小时内同样敏感。在红辐射抑制后（6530 ~ 6635 A）萌发对红辐射的促进作用和7000 A以上辐照对抑制萌发的作用同样敏感。一种对温度敏感的白籽莴苣品种的种子，如果在30℃的黑暗环境中保存几天，如果回到20℃的黑暗环境中，种子仍然处于休眠状态，但是，如果在回到20℃的黑暗环境之前，在5800至6800℃的黑暗环境中照射，则可以促进完全发芽。对5200 A以下蓝辐射作用谱的初步研究表明，蓝辐射能有效促进发芽。
The action spectrum for promotion and inhibition of germination of Grand Rapids lettuce seed was obtained with a 2-prism (glass) spectro-graph having a dispersion of about 35 A/cm. at 6000 A. The slit width was about 90 A at this wavelength and the incident energy 0.100 x 10-3 joules/cm2./sec. Lettuce seeds were placed on wet blotters in dishes and maintained in darkness at 20[degree] C for various times before irradiation. Those to be inhibited in germination were exposed to radiation between 5800 and 6600 A (which gave 98+% germination in the controls), after which they were irradiated in various wavelength regions to test for inhibition. Those to be promoted in germination were taken directly from darkness and irradiated. After irradiation all seeds were held for 2 days at 20oC in darkness before germination counts were made. The seed was equally sensitive for 4 to 16 hrs. after imbibition to promotion by red radiation (between 6530 and 6635 A) and equally sensitive for the same period of imbibition to suppression o germination by irradiation at more than 7000 A. Seeds of a temp.- sensitive white-seeded lettuce var., if held for several days in darkness at 30oC, remained dormant if returned to 20[degree] in darkness, but, if irradiated at 5800 to 6800 A before returning to 20[degree] in darkness, full germination was promoted. Preliminary studies on the action spectrum below 5200 A indicated that blue radiation could effectively promote germination.