Is there a telomere-bound 'EST' telomerase holoenzyme?
Is there a telomere-bound 'EST' telomerase holoenzyme?
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DOI：
10.4161/cc.9.10.11536
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发表时间：
2010-05-15
期刊：
影响因子：
4.3
通讯作者：
Freeman, Brian C.
中科院分区：
文献类型：
--
作者：
DeZwaan, Diane C.;Freeman, Brian C.
真核线性染色体最终形成称为端粒的核蛋白结构。末端端粒 DNA 由富含 G 基序的串联重复组成，该基序是通过专门的逆转录酶端粒酶的作用建立和维持的。除了核心酶之外，端粒DNA的有效复制还需要许多调控因素。在芽殖酵母中，在开创性的更短端粒 (EST) 遗传筛选中鉴定出的四种成分构成了维持端粒 DNA 的关键蛋白。重要的是，从酵母到人类，EST 蛋白在结构上似乎都是保守的。本综述讨论了端粒酶募集和调节的机制，重点是 EST 蛋白。
Eukaryotic linear chromosomes culminate in nucleoprotein structures designated telomeres. The terminal telomeric DNA consists of tandem repeats of a G-rich motif that is established and maintained by the action of the specialized reverse transcriptase telomerase. In addition to the core enzyme, effective replication of telomeric DNA requires a number of regulatory factors. In budding yeast, four components identified in the seminal Ever Shorter Telomere (EST) genetic screens constitute the keystone proteins that sustain telomeric DNA. Importantly, the EST proteins appear to be structurally conserved from yeast to human. The mechanism of telomerase recruitment and regulation, with an emphasis on the EST proteins, are discussed in this review.