Trefoil factor 3 overexpression in prostatic carcinoma: Prognostic importance using tissue microarrays
Trefoil factor 3 overexpression in prostatic carcinoma: Prognostic importance using tissue microarrays
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DOI：
10.1002/pros.20095
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发表时间：
2004-11-01
期刊：
影响因子：
2.8
通讯作者：
De Marzo, AM
中科院分区：
文献类型：
--
作者：
Faith, DA;Isaacs, WB;De Marzo, AM
背景。人肠三叶因子3 （TFF3）是由21号染色体上的一组基因编码的一个多肽家族的成员。通过基因表达谱研究发现TFF3 mRNA在前列腺癌中过表达。我们使用组织微阵列免疫化学和软件工具（统称为TMAJ）在线评估染色，分析了294例原发肿瘤和61例转移性病变样本。以20%的细胞染色为阳性为截止值，正常细胞染色频率为18.8%（272例中有51例），原发肿瘤细胞染色频率为47.0%（268例中有126例），P < 0.0001 （Wilcoxon秩和）。TFF3在转移性前列腺癌中的表达与原发肿瘤相似。TFF3的表达与前列腺癌的生化复发时间、远处转移的发展或死亡无关。来自视觉估计表达的评分数据与使用Chromavision公司的自动细胞成像系统（ACIS(TM)）进行的半自动图像分析高度相关。这些研究证实了TFF3在原发性和转移性前列腺癌的一个子集中在蛋白水平上过表达，显示了TMAJ数据库的首次使用，并证明了半自动扫描和免疫组织化学染色组织微阵列切片评分的能力。(C) 2004 Wiley-Liss, Inc。
BACKGROUND. Human intestinal trefoil factor 3 (TFF3) is a member of a family of polypeptides encoded by a cluster of genes on chromosome 21. Through gene expression profiling studies TFF3 mRNA has been found to be overexpressed in prostate cancer.METHODS. We used immunochemistry on tissue microarrays and software tools, collectively referred to as TMAJ, for online assessment of staining to analyze samples from 294 primary tumors and 61 metastatic lesions.RESULTS. Applying a cutoff of 20% of cells staining as positive, the frequency of staining was 18.8% in normal (51 of 272) and 47.0% in primary tumors (126 of 268), P < 0.0001, Wilcoxon rank sum). Expression of TFF3 in metastatic prostate cancer was similar to that in primary tumors. TFF3 expression was not associated with time to biochemical recurrence, development of distant metastasis, or death due to prostate cancer. Scoring data derived from visual estimation of expression correlated highly with semi-automated image analysis using the Automated Cellular Imaging System (ACIS(TM)) from Chromavision, Inc.CONCLUSIONS. These studies validate that TFF3 is overexpressed at the protein level in a subset of primary and metastatic prostate cancers, show the first use of the TMAJ database, and demonstrate the ability to semi-automatically scan and score immunohistochemically stained tissue microarray slides. (C) 2004 Wiley-Liss, Inc.