Redox state ol the cellular free NAD pools, phosphorylation state of the adenylate system and the (Na+‐K+)‐stimulated ATP‐ase in rat liver
Redox state ol the cellular free NAD pools, phosphorylation state of the adenylate system and the (Na+‐K+)‐stimulated ATP‐ase in rat liver
复制标题
大鼠肝脏中细胞游离 NAD 池的氧化还原状态、腺苷酸系统的磷酸化状态和 (Na+-K+) 刺激的 ATP-ase
DOI：
--
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
I. Hassinen
中科院分区：
文献类型：
--
作者：
V. Kinnula;I. Hassinen
研究了缺氧（10％或8％O2 30分钟）对大鼠肝脏细胞质和线粒体磷酸化状态和氧化还原状态的影响。测量对象包括正常动物和暴露于低氧分压（50.5 kPa 或 40.8 kPa 空气）1 天或 7 天的动物。在急性缺氧和缺氧1天或7天后，肝脏中的胞质游离NAD均减少，即乳酸/丙酮酸和sn-甘油-3-磷酸/二氧丙酮磷酸的比率显着增加。仅在急性缺氧时，线粒体游离 NAD 库才会显着减少，根据肝脏羟基丁酸/乙酰乙酸比值的评估，在 40.8 kPa 压力下保持 1 天或 7 天的动物中，仅观察到轻微减少。肝脏 ATP 浓度在急性缺氧时迅速下降，在 40.8 kPa 压力下的 1 或 7 天内没有任何显着恢复。肝脏 ATP/ADP X Pi 比率显着下降，同时总腺嘌呤核苷酸浓度下降。观察到 7 天后 ATP/ADP X Pi 比率有恢复正常的趋势，即急性缺氧时的值明显小于 7 天后缺氧时的值，表明长时间缺氧期间能量代谢的适应。肝脏 (Na+-K+) 刺激的 ATP 酶活性不受缺氧影响。
The effect of hypoxia (30 min 10% or 8% O2) on the phosphorylation state and redox state of the cytosol and mitochondria of rat liver were studied. Measurements were made both from normal animals and animals which had been exposed to the reduced partial pressure of oxygen (50.5 kPa or 40.8 kPa of air) for one or seven days. Cytostolic free NAD was reduced in the liver both in acute hypoxia and in hypoxia after one or seven days, i.e. the lactate/pyruvate and sn-glycerol-3-phosphate/dioxyacetonephosphate ratios increased markedly. A marked reduction in the mitochondrial free NAD pool occurred only in acute hypoxia and only a slight reduction was observed in animals kept at 40.8 kPa for one or seven days, as evaluated from the hepatic hydroxybutyrate/acetoacetate ratio. Liver ATP concentration decreased rapidly in acute hypoxia without any significant recovery during one or seven days at 40.8 kPa. The hepatic ATP/ADP X Pi ratio decreased significantly, with a simultaneous decrease in the total adenine nucleotide concentration. A tendency was observed for the ATP/ADP X Pi ratio to return to normal after seven days, i.e. the values in acute hypoxia were significantly smaller than those noted in hypoxia after seven days, demonstrating an adaptation of the energy metabolism during prolonged hypoxia. Hepatic (Na+-K+)-stimulated ATP-ase activity was not affected by hypoxia.