Induction of mosquito hemolymph proteins in response to immune challenge and wounding
Induction of mosquito hemolymph proteins in response to immune challenge and wounding
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DOI：
10.1016/s0145-305x(99)00047-6
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发表时间：
1999-10-01
影响因子：
2.9
通讯作者：
Paskewitz, SM
中科院分区：
文献类型：
--
作者：
Han, YS;Chun, JS;Paskewitz, SM
用免疫诱导剂（大肠杆菌、脂多糖、海带多糖、酵母多糖）对冈比亚按蚊（Anopheles gambiae）致伤或注射后血淋巴中蛋白质的快速诱导进行了研究。一维凝胶电泳显示，至少有六个血淋巴多肽>25 kDa，始终出现后，任何臀的角质层。所有这些多肽在30分钟内出现在血淋巴中，并在大约30分钟后达到最大浓度。6小时没有蛋白质被细菌或细菌或真菌细胞壁产物特异性诱导，然而两种组成型表达的蛋白质被这些注射抑制。通过双向电泳进一步分析血淋巴蛋白的模式。在四次重复实验中，注射后2小时增强或诱导了七个斑点。另外两个斑点在重复样品之间表现出一定的变异性，但通常对进样有响应。这些快速诱导的多肽是调节和启动蚊子对病原体和创伤的反应的候选物。(C)1999爱思唯尔科技有限公司。保留所有权利。
The rapid induction of proteins in the hemolymph of the mosquito, Anopheles gambiae, was examined after wounding or injection of immune elicitors (Escherichia coli, lipopolysaccharide, laminarin, zymosan). One-dimensional gel electrophoresis revealed at least six hemolymph polypeptides >25 kDa that consistently appeared after any breech of the cuticle. All of these polypeptides appeared in the hemolymph within 30 min and reached a maximum concentration after approx. 6 h. No proteins were specifically induced by bacteria or bacterial or fungal cell wall products, however two constitutively expressed proteins were repressed by these injections. Patterns of hemolymph proteins were further analyzed by two-dimensional electrophoresis. Seven spots were enhanced or induced 2 h after injection in four replicate experiments. An additional two spots demonstrated some variability between replicates, but were generally responsive to injection. These rapidly induced polypeptides are candidates for regulating and initiating the mosquito's responses to pathogens and wounding. (C) 1999 Elsevier Science Ltd. All rights reserved.