A Phaseolus lectin anterograde tracing study of the tectorotundal projections in the domestic chick.
A Phaseolus lectin anterograde tracing study of the tectorotundal projections in the domestic chick.
复制标题
家鸡顶盖圆投影的菜豆凝集素顺行追踪研究。
作者：
T. Ngo;D. Davies;G. Egedi;T. Tömböl
通过将顺行示踪剂菜豆凝集素 (PHA-L) 离子导入视顶盖并通过高尔基体浸渍来研究从鸡视顶盖到圆核的投射。在同侧圆核内观察到 PHA-L 标记的纤维，并穿过视上交错到达对侧圆核。它们看起来与高尔基浸渍材料中明显的纤维相似。在到达圆核之前，PHA-L 标记的纤维在丘脑尾腹核中发出短的末端分支。在同侧圆核内，标记的纤维形成广泛的纤维网，具有致密的核心和不太致密的外围。对侧圆核中的 PHA-L 标记纤维表现出质量相似但分布较稀疏的情况。圆核内似乎有视顶盖的地形表征。电子显微镜显示，PHA-L 标记的顶盖纤维与圆核中的主要神经元建立了不对称突触。
The projection from the chick optic tectum to the nucleus rotundus was investigated by iontophoresis of the anterograde tracer Phaseolus vulgaris lectin (PHA-L) into the optic tectum and by Golgi impregnation. PHA-L labelled fibres were observed within the ipsilateral nucleus rotundus and to course through the supraoptic decussation to the contralateral nucleus rotundus. They appeared similar to fibres evident in Golgi impregnated material. Before reaching the nucleus rotundus, PHA-L labelled fibres gave off short terminal branches in the caudoventral thalamic nucleus. Within the ipsilateral nucleus rotundus, the labelled fibres formed an extensive fibre net with a dense core and a less dense periphery. PHA-L labelled fibres in the contralateral nucleus rotundus exhibited a qualitatively similar but sparser distribution. There appeared to be a topographic representation of the optic tectum within the nucleus rotundus. Electron microscopy revealed that PHA-L labelled tectal fibres established asymmetric synapses with principal neurons in the nucleus rotundus.