Array‐Based Discrimination of Phenolic Acids in Water at Near‐Neutral pH with Chromogenic and Fluorescent Cyclodextrin‐Based Supramolecular Sensors
Array‐Based Discrimination of Phenolic Acids in Water at Near‐Neutral pH with Chromogenic and Fluorescent Cyclodextrin‐Based Supramolecular Sensors
复制标题
使用基于显色和荧光环糊精的超分子传感器对近中性 pH 条件下水中的酚酸进行基于阵列的辨别
DOI：
10.1002/anse.202200057
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bonizzoni, Marco
中科院分区：
文献类型：
--
作者：
Yao, Xiyuan;Bonizzoni, Marco
酚酸是一类难溶于水的有机化合物，具有抗氧化特性，含有一种羧酸和一种酚基，在植物中很常见。我们使用羟丙基-β-环糊精(HP-β-CD)作为超分子宿主，在生理pH附近检测水中的酚酸。通过一系列六种有机染料的光吸收和发射性能的变化来监测分析物的络合情况，这些有机染料是从17个候选者的筛选小组中挑选出来的，作为置换试验的指示剂。这些染料与HP-β-CD的结合常数在102-104范围内。我们发现，当使用主成分分析(PCA)等多变量分析算法分析和总结与这种置换过程相关的染料光学特征的细微差别时，可以成功地区分六种酚酸，并识别这种酸的六个未知样品，精确度为0.02 mm。
Phenolic acids are a class of poorly water‐soluble organic compounds with antioxidant properties that contain a carboxylic acid and a phenol group, common in plants. We used hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) as a supramolecular host to sense phenolic acids in water near physiological pH. The analytes’ complexation was monitored through changes in the optical absorption and emission properties of a series of six organic dyes, acting as indicators in a displacement assay, that were selected from screening a panel of 17 candidates. Binding constants between these dyes and the HP‐β‐CD were in the 102–104range. We showed that the nuanced differences in the dyes’ optical signatures associated with this displacement process, when analyzed and summarized using multivariate analysis algorithms such as principal component analysis (PCA), can be used to successfully discriminate among six phenolic acids, and to identify six unknown samples of such acids, down to a 0.02 mM concentration.