Rapid and Sensitive Detection of Bovine Coronavirus and Group A Bovine Rotavirus from Fecal Samples by Using One-Step Duplex RT-PCR Assay
Rapid and Sensitive Detection of Bovine Coronavirus and Group A Bovine Rotavirus from Fecal Samples by Using One-Step Duplex RT-PCR Assay
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DOI：
10.1292/jvms.10-0401
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发表时间：
2011-04-01
影响因子：
1.2
通讯作者：
Sueyoshi, Masuo
中科院分区：
文献类型：
--
作者：
Zhu, Wei;Dong, Jianbao;Sueyoshi, Masuo
牛冠状病毒（BCoV）和A组牛轮状病毒（BRV）是引起新生犊牛腹泻的两种主要病原。本研究建立了一步法双重RT-PCR检测和区分粪便标本中BCoV和A组BRV的方法。该方法对BCoV和A组BRV的灵敏度分别为10 PFU/100 μ l和1 PFU/100 μ l。用该方法检测28份腹泻粪便标本，结果2份标本为BCoV和A群BRV共感染，26份标本为A群BRV阳性，2份标本为阴性。结果表明，该方法对临床粪便标本的检测灵敏度高，可用于BCoV和A组BRV的实验室诊断。
Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 mu l and I PFU/100 mu l, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV.