INDUCTION OF AN OUTER SURFACE PROTEIN ON BORRELIA-BURGDORFERI DURING TICK FEEDING
INDUCTION OF AN OUTER SURFACE PROTEIN ON BORRELIA-BURGDORFERI DURING TICK FEEDING
复制标题
DOI：
10.1073/pnas.92.7.2909
复制
发表时间：
1995-03-28
影响因子：
11.1
通讯作者：
ROSA, PA
中科院分区：
文献类型：
--
作者：
SCHWAN, TG;PIESMAN, J;ROSA, PA
莱姆病螺旋体，广义伯氏疏螺旋体，维持在涉及蜱和小型哺乳动物的人畜共患循环中。在未进食的蜱中，螺旋体产生一种外表面蛋白OspA，但不产生OspC。在哺乳动物感染期间，免疫学数据表明螺旋体已经改变了它们的表面，现在表达OspC，但很少或不表达OspA。我们通过体外生长实验发现，这种变化部分受到温度的调节; OspC在32-37摄氏度下由螺旋体产生，但在24摄氏度下则不然。此外，蜱虫中肠中的螺旋体在小鼠身上完全充血，现在它们的表面上有OspC。因此，两个环境线索，温度升高和蜱虫进食，触发螺旋体外膜的重大改变。蜱虫取食期间螺旋体快速合成OspC可能在这些细菌通过蜱虫传播时成功感染哺乳动物宿主（包括人类）的能力中发挥重要作用。
Lyme disease spirochetes, Borrelia burgdorferi sensu lato, are maintained in zoonotic cycles involving ticks and small mammals. In unfed ticks, the spirochetes produce one outer surface protein, OspA, but not OspC. During infection in mammals, immunological data suggest that the spirochetes have changed their surface, now expressing OspC but little or no OspA. We find by in vitro growth experiments that this change is regulated in part by temperature; OspC is produced by spirochetes at 32-37 degrees C but not at 24 degrees C. Furthermore, spirochetes in the midgut of ticks that have fully engorged on mice now have OspC on their surface. Thus two environmental cues, an increase in temperature and tick feeding, trigger a major alteration of the spirochetal outer membrane. This rapid synthesis of OspC by spirochetes during tick feeding may play an essential role in the capacity of these bacteria to successfully infect mammalian hosts, including humans, when transmitted by ticks.