Differentiation of epithelial cells to M cells in response to bacterial colonization on the follicle-associated epithelium of Peyer's patch in rat small intestine
Differentiation of epithelial cells to M cells in response to bacterial colonization on the follicle-associated epithelium of Peyer's patch in rat small intestine
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DOI：
10.1292/jvms.68.1023
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发表时间：
2006-10-01
影响因子：
1.2
通讯作者：
Kitagawa, Hiroshi
中科院分区：
文献类型：
--
作者：
Chin, Keigi;Onishi, Sachiko;Kitagawa, Hiroshi
为阐明M细胞与肠道菌群的关系，在生理条件下，对大鼠Peyer集合淋巴结内细菌定植及向M细胞分化进行了组织平面学研究。滤泡相关上皮（FAE）除顶区狭窄外，被邻近的肠绒毛和一层厚的粘膜层紧密覆盖，后者也充满了绒毛间隙以及FAE与邻近肠绒毛之间的间隙。土著细菌坚持几乎不断的狭窄地区的顶端区域的肠绒毛和FAE。细菌菌落偶尔位于FAE的基部至中部区域，其中M细胞也出现，形成大口袋。当细菌菌落位于FAE的基部至中部区域时，具有相同形态特征的细菌也在邻近Peyer集合淋巴结的绒毛间隙中增殖。然而，在FAE基底至中部区域没有细菌菌落的情况下，FAE中M细胞很少。组织平面分析显示FAE和M细胞上的细菌菌落分布模式相似。M细胞在超微结构上吞噬了土著细菌，然后将其运送到口袋中。这些结果表明，在FAE上的土著细菌定殖在生理条件下刺激FAE中M细胞的分化。M细胞对细菌的摄取可能有助于调节小肠内土著细菌菌落的发育。
To clarify the relationship between M cells and intestinal microflora, histoplanimetrical investigation into the bacterial colonization and the differentiation to M cells was carried out in rat Peyer's patch under physiological conditions. The follicle-associated epithelium (FAE), except for the narrow area of apical region, was closely covered with both neighboring intestinal villi and a thick mucous layer, the latter of which also filled the intervillous spaces as well as the space between the FAE and the neighboring intestinal villi. Indigenous bacteria adhered almost constantly to the narrow areas of apical regions of both intestinal villi and the FAE. Bacterial colonies were occasionally located on the basal to middle region of FAE, where M cells also appeared, forming large pockets. When bacterial colonies were located on the basal to middle region of FAE, bacteria with the same morphological characteristics also proliferated in the intervillous spaces neighboring the Peyer's patch. In cases with no bacterial colonies on the basal to middle region of FAE, however, M cells were rare in the FAE. Histoplanimetrical analysis showed the similar distribution pattern of bacterial colonies on the FAE and M cells in the FAE. M cells ultrastructurally engulfed indigenous bacteria, which were then transported to the pockets. These results suggest that indigenous bacterial colonization on the FAE stimulates the differentiation of M cells in the FAE under physiological conditions. The uptake of bacteria by M cells might contribute the regulation of the development of indigenous bacterial colonies in the small intestine.