Tween 20 soluble proteins of Mycoplasma hyopneumoniae as antigen for an enzyme linked immunosorbent assay.
Tween 20 soluble proteins of Mycoplasma hyopneumoniae as antigen for an enzyme linked immunosorbent assay.
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将猪肺炎支原体的 20 种可溶性蛋白作为酶联免疫吸附测定的抗原。
DOI：
10.1016/s0034-5288(18)32632-8
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发表时间：
1980
影响因子：
2.4
通讯作者：
S. Bruggmann
中科院分区：
文献类型：
--
作者：
J. Nicolet;P. Paroz;S. Bruggmann
本文报道了猪肺炎支原体抗原的制备方法，该抗原经中性洗涤剂吐温20处理后，经Sephadex G 25柱层析纯化。通过酶联免疫吸附试验测定抗原活性和特异性，并与全细胞SDS溶解的抗原进行比较。Tween 20纯化的抗原显示出相当的活性，但具有更高的特异性。
The preparation of an antigen of Mycoplasma hyopneumoniae containing selected proteins after treatment with the neutral detergent Tween 20 and purification on Sephadex G25 is described. The antigenic activity and the specificity were determined by means of an enzyme linked immunosorbent assay and compared with that of a whole-cell, SDS-solubilised antigen. The Tween 20 purified antigen showed a comparable activity but a higher specificity.