Estrous cyclicity and ovarian follicles in female rats after prenatal exposure to 3,3′,4,4′,5-pentachlorobiphenyl
Estrous cyclicity and ovarian follicles in female rats after prenatal exposure to 3,3′,4,4′,5-pentachlorobiphenyl
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DOI：
10.1016/s0378-4274(03)00175-9
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发表时间：
2003-08-28
影响因子：
3.5
通讯作者：
Furusato, M
中科院分区：
文献类型：
--
作者：
Muto, T;Imano, N;Furusato, M
研究了胎儿期接触PCB126后动情周期和卵泡的变化。雌性SD大鼠于妊娠后第13~19天分别灌胃(Igg)PCB126/kg或赋形剂25pg、2.5、250和7.5杯。250毫微克和7.5毫微克的子代的阴道开放(VO)显著延迟。VO后各组动物均表现出不规则的动情周期，但几天后恢复正常。然而，2.5、250和7.5MUG组在VO后开始正常发情周期的时间明显延迟。在30日龄和50日龄时，2.5、250和7.5 mg组的有腔卵泡数显著少于对照组，而闭锁卵泡数显著高于对照组。在50日龄时，7.5杯组的黄体明显减少。在50天龄的仔鼠中，2.5、250和7.5 ng组的血清17β-雌二醇和孕酮水平显著低于赋形剂组，而脂肪组织中PCB126的水平显著高于赋形剂组。因此，尽管研究中使用的产前剂量的PCB126没有导致外生殖器畸形或持续性卵巢中断，但在暴露于17.5 ng/kg PCB126的母鼠所生的2.5 ng组幼鼠中，发现青春期卵巢功能中断。本研究表明，PCB126至少部分地对卵巢产生了直接影响，表现为动情周期的中断。(C)2003爱思唯尔爱尔兰科学有限公司。保留所有权利。
Alterations of estrous cyclicity and ovarian follicles following prenatal exposure to PCB126 were examined. Female SD rats were given (i.g.) 25 pg, 2.5, 250 ng and 7.5 mug of PCB126/kg or the vehicle on days 13-19 postconception. Vaginal opening (VO) in the 250 ng and 7.5 mug offspring was significantly delayed. All groups showed irregular estrous cyclicity following VO, but it became normal after a few days. However, the start of normal estrous cyclicity following VO in the 2.5, 250 ng and 7.5 mug groups was significantly delayed. At 30 and 50 days old, the 2.5, 250 ng and 7.5 mug groups showed significantly fewer antral follicles and a higher number of atretic follicles. The 7.5 mug group at 50 days old revealed significantly fewer corpus luteums. In 50-day-old offspring, the 2.5, 250 ng and 7.5 mug groups showed a significant reduction in serum 17beta-estradiol and progesterone levels and significantly higher levels of PCB126 in the fatty tissue compared with the vehicle group. Thus, while the prenatal dose of PCB126 used in this study did not induce malformation of the external genitalia or persistent ovarian disruption, disruption of ovarian function at puberty was found in the 2.5 ng group of pups born to dams exposed to 17.5 ng/kg PCB126. The present study suggests that PCB126, at least in part, exerted direct effects on the ovary as shown by the disruption of estrous cyclicity. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.