Gene Expression Profiling of Vasoregression in the Retina-Involvement of Microglial Cells
Gene Expression Profiling of Vasoregression in the Retina-Involvement of Microglial Cells
复制标题
DOI：
10.1371/journal.pone.0016865
复制
发表时间：
2011-02-17
期刊：
影响因子：
3.7
通讯作者：
Hammes, Hans-Peter
中科院分区：
文献类型：
--
作者：
Feng, Yuxi;Wang, Yumei;Hammes, Hans-Peter
血管退化是血管性眼病的一个标志，但涉及的机制仍然在很大程度上未知。我们最近的特点是大鼠纤毛病变模型，发展初级感光细胞变性和继发性血管退行性变。为了提高对视网膜神经变性继发性血管退行性变的理解，我们使用微阵列技术比较了视网膜血管退行性变前后这种新模型中的基因表达谱。通过定量RT-PCR、Western blot和免疫荧光等方法验证差异基因表达。在157个基因的调节超过两倍，MHC II类不变链CD 74产生最强的上调，并分配到激活的小胶质细胞接近血管退行性血管。通路聚类鉴定了免疫系统的基因，包括炎症信号传导，以及在血管消退期间上调的补体级联的组分。总之，我们的数据表明，参与视网膜免疫反应的小胶质细胞参与视网膜血管退行性病变的发生。
Vasoregression is a hallmark of vascular eye diseases but the mechanisms involved are still largely unknown. We have recently characterized a rat ciliopathy model which develops primary photoreceptor degeneration and secondary vasoregression. To improve the understanding of secondary vasoregression in retinal neurodegeneration, we used microarray techniques to compare gene expression profiles in this new model before and after retinal vasoregression. Differential gene expression was validated by quantitative RT-PCR, Western blot and immunofluorescence. Of the 157 genes regulated more than twofold, the MHC class II invariant chain CD74 yielded the strongest upregulation, and was allocated to activated microglial cells close to the vessels undergoing vasoregression. Pathway clustering identified genes of the immune system including inflammatory signaling, and components of the complement cascade upregulated during vasoregression. Together, our data suggest that microglial cells involved in retinal immune response participate in the initiation of vasoregression in the retina.