The Dorsal Raphe Regulates the Duration of Attack through the Medial Orbitofrontal Cortex and Medial Amygdala.
The Dorsal Raphe Regulates the Duration of Attack through the Medial Orbitofrontal Cortex and Medial Amygdala.
复制标题
中缝背侧通过内侧眶前皮质和内侧杏仁核调节发作持续时间。
DOI：
10.1523/eneuro.0331-20.2020
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发表时间：
2020-09-01
期刊：
影响因子：
3.4
通讯作者：
Li, Zheng
中科院分区：
文献类型：
--
作者：
Nordman, Jacob;Li, Zheng
中缝背侧 (DR) 是一种进化上保守的大脑结构，与攻击行为有关。它投射到许多潜在攻击行为的皮质和边缘区域。然而，DR 调节攻击行为的具体神经回路目前尚不清楚。在这项研究中，我们发现表达 CaMKIIalpha 的 DR 神经元会被小鼠的攻击行为激活。这些神经元投射到眶额皮质（OFC；MeOC）的内侧和内侧杏仁核（MeA），这是神经回路中已知控制攻击行为的两个关键区域。使用体内光遗传学方法，我们发现通过抑制 DR 中的 CaMKIIalpha+ 神经元及其在 MeOC 处的轴突可以缩短攻击发作时间，并通过在攻击期间刺激 DR-MeOC 轴突来延长攻击发作时间。相比之下，刺激 MeA 处 CaMKIIalpha+ DR 神经元的轴突可缩短发作时间。值得注意的是，DR-MeOC 或 DR-MeA 途径在受到刺激时都不会发起攻击。这些结果表明DR-MeOC和DR-MeA通路以相反的方向调节攻击行为的持续时间，揭示了DR控制攻击的电路机制。
The dorsal raphe (DR) is an evolutionarily conserved brain structure that is involved in aggressive behavior. It projects onto numerous cortical and limbic areas underlying attack behavior. The specific neurocircuit through which the DR regulates aggression, however, is largely unclear. In this study we show that DR neurons expressing CaMKIIalpha are activated by attack behavior in mice. These neurons project to the medial aspect of the orbitofrontal cortex (OFC; MeOC) and the medial amygdala (MeA), two key regions within the neural circuit known to control aggressive behavior. Using an in vivo optogenetic approach, we show that attack bouts are shortened by inhibiting CaMKIIalpha+ neurons in the DR and their axons at the MeOC and prolonged by stimulating the DR-MeOC axons during an attack. By contrast, stimulating the axons of CaMKIIalpha+ DR neurons at the MeA shortens attack. Notably, neither the DR-MeOC or DR-MeA pathway initiates attack when stimulated. These results indicate that the DR-MeOC and DR-MeA pathways regulate the duration of attack behavior in opposite directions, revealing a circuit mechanism for the control of attack by the DR.