Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system
Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system
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DOI：
10.1099/00207713-48-2-469
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发表时间：
1998-04-01
期刊：
影响因子：
--
通讯作者：
Gjerdevik, K
中科院分区：
文献类型：
--
作者：
Lien, T;Madsen, M;Gjerdevik, K
从北海某石油平台油水分离系统中分离到一株嗜温、革兰氏阴性、棒状、海洋性、丙酸盐氧化硫酸盐还原菌M16（T）。最适生长条件为31 ℃、pH 6.8-7.2、1.5-2.0%（w/v）NaCl和0.1-0.3%（w/v）MgCl 2。6H（2）O。用硫酸盐的生长产量为4.6g细胞生物质（氧化的丙酸盐摩尔数）（-1）。菌株M16（T）在营养上与脱硫菌属的成员相关，但不同之处在于它不需要维生素，并且能够利用富马酸盐和苹果酸盐作为碳源和能源。氢酶活性测定为氢吸收主要是膜结合，并与生长基质。在用氢气生长的细胞中发现最高活性[28 μ mol min（-1）（mg蛋白质）（-1）]，在用丙酸盐作为硫酸盐还原电子供体生长的细胞中发现最低活性[50 nmol min（-1）（mg蛋白质）（-1）]。存在Desulforubidin、甲基萘醌-5（H-2）和c型和b型细胞色素。脂肪酸组成与丙酸脱硫菌相似，DNA碱基组成为50.6mol%G +C。菌株M16（T）与D. propionicus和长叶脱硫泡菌Desulfobulbuselongatus的16 SrDNA同源性为96.1%。根据基因型、表型和免疫学特征的差异，提出菌株M16（T）（= DSM 8777（T））为一新种--弹状脱硫菌的模式菌株。
A mesophilic, Gram-negative, rod-shaped, marine, propionate-oxidizing sulfate reducer (strain M16(T)) was isolated from a water-oil separation system on a North Sea oil platform. The optimum conditions for growth were 31 degrees C, pH 6.8-7.2 and 1.5-2.0%(w/v) NaCl and 0.1-0.3% (w/v) MgCl2 . 6H(2)O in the medium. The growth yield with sulfate was 4.6 g cell biomass (mol propionate oxidized)(-1). Strain M16(T) is nutritionally related to members of the genus Desulfobulbus, but differs in that it has no vitamin requirement and is able to utilize fumarate and malate as carbon and energy sources. Hydrogenase activity measured as hydrogen uptake was mainly membrane-bound and varied with the growth substrate. Highest activity [28 mu mol min(-1) (mg protein)(-1)] was found in cells grown with hydrogen and lowest [50 nmol min(-1) (mg protein)(-1)] in cells grown with propionate as electron donors for sulfate reduction. Desulforubidin, menaquinone-5(H-2) and cytochromes of the c- and b-type were present. The fatty acid pattern was similar to that found for Desulfobulbus propionicus, The DNA base composition was 50.6 mol% G+C. Strain M16(T) is equidistantly related to D. propionicus and Desulfobulbus elongatus with 96.1% 16S rDNA similarity. On the basis of differences in genotypic, phenotypic and immunological characteristics, strain M16(T) (= DSM 8777(T)) is proposed as the type strain of a new species, Desulfobulbus rhabdoformis.