Twist, a novel oncogene is upregulated in pancreatic cancer:: Clinical implication of Twist expression in pancreatic juice
Twist, a novel oncogene is upregulated in pancreatic cancer:: Clinical implication of Twist expression in pancreatic juice
复制标题
DOI：
10.1002/ijc.22295
复制
发表时间：
2007-04-15
影响因子：
6.4
通讯作者：
Tanaka, Masao
中科院分区：
文献类型：
--
作者：
Ohuchida, Kenoki;Mizumoto, Kazuhiro;Tanaka, Masao
尽管有证据表明Twist是一种高度保守的碱性螺旋-环-螺旋转录因子，是一种新的癌基因，但尚未有报道描述Twist在胰腺癌中的表达。胰腺导管内乳头状黏液性肿瘤（IPMN）和胰腺上皮内瘤变（PanIN）是胰腺癌的前驱病变。为了阐明Twist表达在胰腺癌中的作用，我们使用定量逆转录-聚合酶链反应并使用大块组织检测了Twist在胰腺癌、IPMN和非肿瘤胰腺中的表达（11例癌症，18例IPMN和15例非肿瘤性胰腺），显微切割细胞（来自22个切片的癌，来自19个切片的IPMN，来自6个切片的PanIN，和来自14个切片的胰腺炎影响的上皮细胞）和胰液（16例来自癌症，28例来自IPMN，17例来自胰腺炎）。Twist表达在癌症和IPMN大块组织之间显著不同（p < 0.0001），但在癌症和非肿瘤组织之间没有显著差异。Twist表达在显微切割的癌细胞、IPMN细胞和胰腺炎影响的细胞之间显著不同（所有比较，p < 0.017）。PanIN细胞表达的Twist水平显著低于IDC细胞（p = 0.016）。Twist表达在癌症和IPMN汁液样品之间存在显著差异（p = 0.0002），但在癌症和胰腺炎汁液样品之间没有差异。受试者操作特征曲线分析显示，Twist的测量更有助于区分癌症与IPMN，而不是慢性胰腺炎（p = 0.009）。我们的研究结果表明，扭曲参与胰腺癌的肿瘤进展，并在胰液中的扭曲测量可能是有用的，以区分胰腺癌从非恶性肿瘤，如IPMN。(c)2007 Wiley-Liss，Inc.
Despite evidence that Twist, a highly conserved basic helix-loop-helix transcription factor, is a novel oncogene, there are no reports describing Twist expression in pancreatic cancer. Intraductal papillary mucinous neoplasm (IPMN) and pancreatic intraepithelial neoplasia (PanIN) are precursor lesions of pancreatic cancer. To clarify involvement of Twist expression in pancreatic cancer, we used quantitative reverse transcription-polymerase chain reaction and examined Twist expression in pancreatic cancer, IPMN, and non-neoplastic pancreas using bulk tissues (11 cancers, 18 IPMNs, and 15 non-neoplastic pancreata), microdissected cells (cancer from 22 sections, IPMN from 19 sections, PanIN from 6 sections, and pancreatitis-affected epithelial cells from 14 sections), and pancreatic juice (16 from cancer, 28 from IPMN, and 17 from pancreatitis). Twist expression differed significantly between cancer and IPMN bulk tissues (p < 0.0001) but not between cancer and non-neoplastic tissues. Twist expressions differed significantly between microdissected cancer cells, IPMN cells, and pancreatitis-affected cells (all comparisons, p < 0.017). PanIN cells expressed significantly lower levels of Twist than did IDC cells (p = 0.016). Twist expression differed significantly between cancer and IPMN juice samples (p = 0.0002) but not between cancer and pancreatitis juice samples. Receiver operation characteristic curve analyses revealed that measurement of Twist was more useful for discriminating cancer from IPMN than from chronic pancreatitis (p = 0.009). Our results suggest that Twist is involved in tumor progression of pancreatic cancer and that measurement of Twist in pancreatic juice may be useful to differentiate pancreatic cancer from nonmalignant neoplasms such as IPMN. (c) 2007 Wiley-Liss, Inc.