Measurement of peroxiredoxin activity.
Measurement of peroxiredoxin activity.
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DOI：
10.1002/0471140856.tx0710s49
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发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Parsonage, Derek
中科院分区：
文献类型：
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作者：
Nelson, Kimberly J;Parsonage, Derek
过氧化物还毒素是依赖半胱氨酸的过氧化物酶，可与过氧化氢、较大的过氧化氢底物和过氧亚硝酸盐反应。通过1)与NADPH、硫氧还蛋白还原酶和硫氧还蛋白的偶联反应，2)硫氧还蛋白氧化的直接监测，3)与辣根过氧化物酶的竞争，以及4)使用FOX实验的过氧化物消耗，提供了测量Prx与过氧化物活性的方案。
Peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by 1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, 2) the direct monitoring of thioredoxin oxidation, 3) competition with horseradish peroxidase, and 4) peroxide consumption using the FOX assay.