Structural diversity of pectins isolated from the Styrian oil-pumpkin (Cucurbita pepo var. styriaca) fruit
Structural diversity of pectins isolated from the Styrian oil-pumpkin (Cucurbita pepo var. styriaca) fruit
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DOI：
10.1016/j.carbpol.2012.05.017
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发表时间：
2013-03-01
影响因子：
11.2
通讯作者：
Ebringerova, Anna
中科院分区：
文献类型：
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作者：
Kost'alova, Zuzana;Hromadkova, Zdenka;Ebringerova, Anna
为了从果胶成分的角度评估施蒂里亚油南瓜种子果实的生物量，通过使用热水、EDTA、稀盐酸（两次）和稀且更强的氢氧化钠溶液的六步连续提取来分离一系列酸性多糖。化学，物理化学和光谱分析表明，前四个馏分包括部分甲基酯化和乙酰化果胶与不同比例的半乳糖和阿拉伯糖的侧链分支的鼠李糖半乳糖醛酸区域，并表现出相当大的多分子性。碱提取的多糖含有较低量的果胶，其具有在最后多糖中占主导地位的半纤维素旁边的高半乳糖醛酸聚糖和富含阿拉伯糖的鼠李糖半乳糖醛酸聚糖区域。使用H-1-C-13 HSQC和HMBC光谱，在纯化的酸提取的果胶中的游离和甲基酯化的半乳糖醛酸残基的共振被明确地建立，并且由两个残基形成的各种二联体被鉴定。研究结果可为探索利用油南瓜果实生物质生产果胶的工艺条件提供依据。(C)2012爱思唯尔有限公司保留所有权利。
To evaluate the seeded fruit biomass of the Styrian oil-pumpkin in view of its pectin component, a series of acidic polysaccharides were isolated by a six-step sequential extraction using hot water, EDTA, dilute HCl (twice) and dilute and stronger NaOH solutions. Chemical, physicochemical and spectroscopy analyses revealed that the first four fractions comprised partially methyl-esterified and acetylated pectins with varying proportions of rhamnogalacturonan regions ramified with galactose- and arabinose-containing side chains and showed considerable polymolecularity. The alkali-extracted polysaccharides contained lower amounts of pectins with homogalacturonan and arabinose-rich rhamnogalacturonan regions next to hemicelluloses prevailing in the last polysaccharide. Using H-1-C-13 HSQC and HMBC spectroscopy, the resonances of free and methylesterified galacturonic acid residues in the purified acid-extracted pectin were unambiguously established and various diads formed by both residues identified. The results might serve as a basis for searching technological conditions to produce pectin from the oil-pumpkin fruit biomass. (C) 2012 Elsevier Ltd. All rights reserved.