Multiple determinants controlling activation of yeast replication origins late in S phase
Multiple determinants controlling activation of yeast replication origins late in S phase
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DOI：
10.1101/gad.10.13.1595
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发表时间：
1996-07-01
影响因子：
10.5
通讯作者：
Fangman, WL
中科院分区：
文献类型：
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作者：
Friedman, KL;Diller, JD;Fangman, WL
对酵母XIV染色体左臂从端粒开始157 kb的131-kb片段的分析揭示了四个高度活跃的复制起源，它们在S期后期开始复制。先前的研究表明，端粒是迟发激活的决定因素。然而，当位于大的圆形质粒上时，至少有两个染色体XIV起源保持其较晚的激活时间，这表明晚期复制与端粒无关。对含有不同数量的XIV染色体DNA的质粒衍生物的复制时间的分析表明，至少有三个染色体元素，不同于每个测试的起源，有助于延迟激活时间。这些晚期决定因素在功能上是等价的，因为一组贡献序列的重复可以补偿另一组的删除。此外，插入一个通常早期激活到该区域的起源会导致向晚期激活的转变，这表明XIV染色体起源对晚期决定因子的反应能力并不是唯一的。
Analysis of a 131-kb segment of the left arm of yeast chromosome XIV beginning 157 kb from the telomere reveals four highly active origins of replication that initiate replication late in S phase. Previous work has shown that telomeres act as determinants for late origin activation. However, at least two of the chromosome XIV origins maintain their late activation time when located on large circular plasmids, indicating that late replication is independent of telomeres. Analysis of the replication time of plasmid derivatives containing varying amounts of chromosome XIV DNA show that a minimum of three chromosomal elements, distinct from each tested origin, contribute to late activation time. These late determinants are functionally equivalent, because duplication of one set of contributing sequences can compensate for the removal of another set. Furthermore, insertion of an origin that is normally early activated into this domain results in a shift to late activation, suggesting that the chromosome XIV origins are not unique in their ability to respond to the late determinants.