Comparative effect of 25(OH)D3 and 1,25(OH)2D3 on Th17 cell differentiation
Comparative effect of 25(OH)D3 and 1,25(OH)2D3 on Th17 cell differentiation
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DOI：
10.1016/j.clim.2016.02.011
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发表时间：
2016-05-01
影响因子：
8.6
通讯作者：
Khoury, Samia J.
中科院分区：
文献类型：
--
作者：
Fawaz, Lama;Mrad, May F.;Khoury, Samia J.
维生素D是一种类固醇激素，在钙稳态和骨代谢中起着重要的调节作用。免疫细胞既可以产生1，25(OH)(2)D3，也可以对其做出反应。来自维生素D受体(VDR)KO小鼠的CD4+T细胞比野生型KO小鼠产生更高水平的干扰素-γ和IL-17，在自身免疫性疾病(AID)的发病机制中发挥着关键作用。我们对维生素D对人类致病Th17细胞的影响特别感兴趣。我们研究了1，25(OH)(2)D3和25(OH)D3对正常供者原代CD4+T细胞分化和细胞因子产生的影响，并在Th17极化条件下进行培养。两种形式的维生素D都减少了致病Th17标志物的表达及其促炎细胞因子(IL-17A、干扰素-γ)的分泌。此外，两种形式的维生素D都诱导CD25hi细胞扩张，并上调其CTLA-4和Foxp3调节标志物的表达。(C)2016 Elsevier Inc.保留所有权利。
Vitamin D is a secosteroid hormone that plays an important regulatory role in calcium homeostasis and bone metabolism. Immune cells can both produce and respond to 1,25(OH)(2)D3. CD4+ T cells from vitamin D receptor (VDR) KO mice produce higher levels of IFN-gamma and IL-17 than their wild type counterparts, and play a crucial role in the pathogenesis of autoimmune diseases (AID). We are particularly interested in studying the effect of vitamin D on pathogenic Th17 cells in humans.We investigated the in vitro effect of 1,25(OH)(2)D3 and 25(OH)D3 on the differentiation and cytokine production of primary CD4+ T cells from normal donors, and cultured in Th17 polarizing conditions. Both forms of vitamin D reduced the expression of pathogenic Th17 markers and their secretion of pro-inflammatory cytokines (IL-17A, IFN-gamma). Furthermore, both vitamin D forms induced an expansion of CD25hi cells and upregulated their expression of CTLA-4 and Foxp3 regulatory markers. (C) 2016 Elsevier Inc. All rights reserved.