Lactobacillus kitasatonis sp nov., from chicken intestine
Lactobacillus kitasatonis sp nov., from chicken intestine
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DOI：
10.1099/ijs.0.02815-0
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发表时间：
2003-11-01
影响因子：
2.8
通讯作者：
Ohori, H
中科院分区：
文献类型：
--
作者：
Mukai, T;Arihara, K;Ohori, H
对从鸡小肠分离的4株乳酸杆菌和JCM 1038、JCM 1039（命名为嗜酸乳杆菌）进行了表型和分子分类学研究。它们是革兰氏阳性、过氧化氢酶阴性、不从葡萄糖产生气体的兼性厌氧杆菌。这些菌株具有相似的表型特征，并且表现出> 77%的组间DNA相关性值，表明它们包括单一物种。测定了代表性菌株JCM 1039（T）（在本研究中指定为模式菌株）的16 S rRNA基因序列，并与其他乳杆菌属物种的16 S rRNA基因序列进行了比对。基于系统发育分析，JCM 1039（T）被归入乳酸杆菌属的德氏乳杆菌（Lactobacillus delbrueckii）类群，并形成了一个独立的类群，与其最近的邻群Lactobacillus amylovorus、Lactobacillus crispatus、Lactobacillus gallinarum、L.嗜酸乳杆菌和瑞士乳杆菌。DNA-DNA杂交实验和全细胞蛋白质谱的结果清楚地表明，这些菌株代表了一种新的乳杆菌属物种，为此提出了名称Lactobacillus kitasatonis sp. nov.;该物种的模式菌株是JCM 1039（T）。
Four strains isolated from chicken small intestine and strains JCM 1038 and JCM 1039 (designated as Lactobacillus acidophilus) were characterized by phenotypic and molecular taxonomic methods. They were Gram-positive, catalase-negative, facultatively anaerobic rods that did not produce gas from glucose. These strains had similar phenotypic characteristics and exhibited intergroup DNA relatedness values of >77%, indicating that they comprised a single species. The 16S rRNA gene sequence of a representative strain, JCM 1039(T) (designated as type strain in this study), was determined and aligned with those of other Lactobacillus species. JCM 1039(T) was placed in the Lactobacillus delbrueckii cluster of the genus Lactobacillus on the basis of phylogenetic analysis and formed an independent cluster that was distinct from its closest neighbours, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum, L. acidophilus and Lactobacillus helveticus. Results of DNA-DNA hybridization experiments and whole-cell protein profiles clearly indicated that these strains represent a novel Lactobacillus species, for which the name Lactobacillus kitasatonis sp. nov. is proposed; the type strain of this species is JCM 1039(T).